WormBase Tree Display for Construct: WBCnstr00017007
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WBCnstr00017007 | Other_name | Expr10581_Ex | |
---|---|---|---|
Summary | [dcr-1::GFP] | ||
Driven_by_gene | WBGene00000939 | ||
Fusion_reporter | GFP | ||
Construction_summary | [dcr-1::GFP]. The dcr-1 gene, including all intronic regions, was amplified from the fosmid clone WRM066bH04 (Geneservice) with Phusion High-Fidelity DNA Polymerase (Finnzymes) and the oligos 5'-AGCATGCATGGTCAGGGTAAGAGCTGAT-3' and 5'-ACCCGGGGAACAGTTGTTAATGATGGGC-3', and subcloned into pGem-T Easy vector (Promega). The gene was then transferred to the pPD95.75.pges-1 vector using the Spe I and Xma I sites. The presumptive dcr-1 promoter was obtained by amplification of 0.6 kb of the upstream genomic sequence with the oligos 5' -TAAGCTTAAAACTCACCATCAGGC ATTCT-3' and 5' -ACCCGGGGAACAGTTGTTAATGATGGGC-3' and cloned upstream of dcr-1::GFP insert. | ||
Clone | WRM066bH04 | ||
Used_for | Transgene_construct | WBTransgene00031374 | |
Reference | WBPaper00041520 | ||
Remark | [dcr-1::GFP]. The dcr-1 gene, including all intronic regions, was amplified from the fosmid clone WRM066bH04 (Geneservice) with Phusion High-Fidelity DNA Polymerase (Finnzymes) and the oligos 5'-AGCATGCATGGTCAGGGTAAGAGCTGAT-3' and 5'-ACCCGGGGAACAGTTGTTAATGATGGGC-3', and subcloned into pGem-T Easy vector (Promega). The gene was then transferred to the pPD95.75.pges-1 vector using the Spe I and Xma I sites. The presumptive dcr-1 promoter was obtained by amplification of 0.6 kb of the upstream genomic sequence with the oligos 5' -TAAGCTTAAAACTCACCATCAGGC ATTCT-3' and 5' -ACCCGGGGAACAGTTGTTAATGATGGGC-3' and cloned upstream of dcr-1::GFP insert. |