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WormBase Tree Display for Construct: WBCnstr00017007

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Name Class

WBCnstr00017007Other_nameExpr10581_Ex
Summary[dcr-1::GFP]
Driven_by_geneWBGene00000939
Fusion_reporterGFP
Construction_summary[dcr-1::GFP]. The dcr-1 gene, including all intronic regions, was amplified from the fosmid clone WRM066bH04 (Geneservice) with Phusion High-Fidelity DNA Polymerase (Finnzymes) and the oligos 5'-AGCATGCATGGTCAGGGTAAGAGCTGAT-3' and 5'-ACCCGGGGAACAGTTGTTAATGATGGGC-3', and subcloned into pGem-T Easy vector (Promega). The gene was then transferred to the pPD95.75.pges-1 vector using the Spe I and Xma I sites. The presumptive dcr-1 promoter was obtained by amplification of 0.6 kb of the upstream genomic sequence with the oligos 5' -TAAGCTTAAAACTCACCATCAGGC ATTCT-3' and 5' -ACCCGGGGAACAGTTGTTAATGATGGGC-3' and cloned upstream of dcr-1::GFP insert.
CloneWRM066bH04
Used_forTransgene_constructWBTransgene00031374
ReferenceWBPaper00041520
Remark[dcr-1::GFP]. The dcr-1 gene, including all intronic regions, was amplified from the fosmid clone WRM066bH04 (Geneservice) with Phusion High-Fidelity DNA Polymerase (Finnzymes) and the oligos 5'-AGCATGCATGGTCAGGGTAAGAGCTGAT-3' and 5'-ACCCGGGGAACAGTTGTTAATGATGGGC-3', and subcloned into pGem-T Easy vector (Promega). The gene was then transferred to the pPD95.75.pges-1 vector using the Spe I and Xma I sites. The presumptive dcr-1 promoter was obtained by amplification of 0.6 kb of the upstream genomic sequence with the oligos 5' -TAAGCTTAAAACTCACCATCAGGC ATTCT-3' and 5' -ACCCGGGGAACAGTTGTTAATGATGGGC-3' and cloned upstream of dcr-1::GFP insert.