WormBase Tree Display for Construct: WBCnstr00013752
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| WBCnstr00013752 | Other_name | Expr9155_Ex | |
|---|---|---|---|
| Summary | [slcf-1p::SLCF-1::GFP] | ||
| Driven_by_gene | WBGene00010340 | ||
| Gene | WBGene00010340 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [slcf-1p::SLCF-1::GFP] translational fusion. slcf-1 predicted gene F59F5.1 is present in the fosmid clone WRM0639bB08. This clone was digested with EcoRI to release a 6.1 kb fragment that included 2,410 bp upstream from start codon and 840 bp downstream from stop codon. This fragment was ligated into pBluescript digested with EcoRI. The AleI site located in the multiple cloning site of pBluescript was then eliminated by digestion with SacI. This SacI digestion removed 320 bp from 5 end of the gene and 60 bp from the vector, giving pFS23. GFP sequence was recovered from pPD119.16 (kind gift from Andy Fire laboratory) by digesting with Ecl136II and ligated in frame into pFS23 linearized with AleI. The resulting construct pFS25 contains the slcf-1 gene with GFP inserted just before the stop codon. --precise ends. | ||
| Clone | pPD119.16 | ||
| Used_for | Transgene_construct | WBTransgene00030935 | |
| Reference | WBPaper00037741 |
