WormBase Tree Display for Construct: WBCnstr00012474
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| WBCnstr00012474 | Other_name | Expr649_Ex | |
|---|---|---|---|
| Summary | [gly-12::lacZ] | ||
| Driven_by_gene | WBGene00001637 | ||
| Fusion_reporter | LacZ | ||
| Construction_summary | [gly-12::lacZ] promoter fusion. A DNA fragment (1740 nt) containing the putative promoter region immediately upstream of the first exon, the complete 5' UTR, and four nucleotide from the open reading frame (the initiation codon ATG was mutated to AAG) was amplified by PCR from the cosmid F54E3 with primers PRFF5 and PRFR6 and subcloned into SalI and BamHI sites of pPD95.57 upstream of the lacZ gene to create plasmid p57F/prom. --precise ends. | ||
| Clone | pPD95.57 | ||
| F54E3 | |||
| Used_for | Transgene_construct | WBTransgene00029682 | |
| Reference | WBPaper00003352 |
