WormBase Tree Display for Construct: WBCnstr00011964
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| WBCnstr00011964 | Other_name | Expr4396_Ex | |
|---|---|---|---|
| Summary | [pps-1::gfp] | ||
| Driven_by_gene | WBGene00004091 | ||
| Gene | WBGene00004091 | ||
| Fusion_reporter | GFP | ||
| EGFP | |||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [pps-1::gfp] translational fusion. The translational pps-1(FL)::egfp reporter construct was generated by PCR amplification of a 5495-bp genomic fragment, including the 3 kb of the potential promoter region. The fragment was amplified by PCR from C. elegans genomic DNA as a template using a 5'-primer (5'-TTTTTATCCCATTTGCCATT-3') and a 3'-primer (5'-GTTTGAGTTTTGTAGTGATTTGTAGTAG-3') located just before the stop codon and ligated into the vector pFX_EGFPT. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00029177 | |
| Reference | WBPaper00027123 |
