WormBase Tree Display for Construct: WBCnstr00011950
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| WBCnstr00011950 | Other_name | Expr4376_Ex | |
|---|---|---|---|
| Summary | [snf-11::gfp] | ||
| Driven_by_gene | WBGene00004910 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [snf-11::gfp] transcriptional fusion. The sense primer (coordinates in cosmid T03F7.1 are 3,896 to 3,925) with an XbaI adapter attached to the 5'-end was 5'-TCT AGA CAT TCG GCG AAG GAT GGT CGA GAG-3', and the reverse primer (coordinates in cosmid T03F7.1 are 5,609 to 5,637) with a BamHI adapter attached to the 5'-end was 5'-GGA TCC CCT GCA GGA GAT TTC TGG ATA CAA-3'. A recombinant KlenTaq DNA polymerase (AB Peptides, St. Louis, MO) was used for the long-range PCR. The size of the expected PCR product is ~2.0 kb. The amplified region contains the putative promoter for the cegat-1 gene. The PCR product was digested with XbaI and BamHI and ligated into multiple cloning sites II in the pPD117.01 vector. --precise ends. | ||
| Clone | pPD117.01 | ||
| Used_for | Transgene_construct | WBTransgene00029163 | |
| Reference | WBPaper00024600 |
