WormBase Tree Display for Construct: WBCnstr00011666
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| WBCnstr00011666 | Other_name | Expr3803_Ex | |
|---|---|---|---|
| Summary | [trx-1::gfp] | ||
| Driven_by_gene | WBGene00015062 | ||
| Gene | WBGene00015062 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [trx-1::gfp] translational fusion. A translational GFP fusion construct was obtained by PCR amplification of the trx-1 promoter and gene from C. elegans genomic DNA. The forward primer B0228.5-fGFP 5$(B!l(B-CATTCTGCAGAGAATGGATACCTGATCATT-3 and the reverse primer B0228.5-rGFP 5$(B!l(B-TCGAGGATCCTTGAGCAGATATCGTGCTCCA-3$(B!l(B were used and the PCR product was cloned into the PstI/BamHI sites of the pPD95.77 vector. MT1642 lin-15(n765ts) X animals were microinjected with the trx-1::gfp fusion construct together with the marker plasmid pBHL98 that rescues the multi-vulva phenotype of the MT1642 strain. --precise ends. | ||
| Clone | pPD95.77 | ||
| Used_for | Transgene_construct | WBTransgene00028882 | |
| Reference | WBPaper00026991 |
