WormBase Tree Display for Construct: WBCnstr00011642
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| WBCnstr00011642 | Other_name | Expr3745_Ex | |
|---|---|---|---|
| Summary | [cgr-1::gfp] | ||
| Driven_by_gene | WBGene00020847 | ||
| Gene | WBGene00020847 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [cgr-1::gfp] translational fusion. A 4.8-kb genomic DNA fragment was used, which includes 1 kb of the cgr-1 promoter region and the complete cgr-1 coding region; the GFP coding region was inserted at the cgr-1 stop codon. A 4785 bp SacI/SacII fragment of cosmid T27A10 that extends 990 bp upstream of the ATG (start) and 760 bp downstream of the TAA (stop) and does not contain sequences from adjacent predicted genes was ligated into pBluescript SK+ (Strategene, La Jolla, CA) to create the plasmid pJG1. pDG125 contains cgr-1 genomic DNA coding region and encodes a CGR-1:GFP fusion protein; it was generated by ligating an 869 bp GFP genomic sequence from pPD95.77 into pJG1 digested with AscI. --precise ends. | ||
| Clone | T27A10 | ||
| pPD95.77 | |||
| Used_for | Transgene_construct | WBTransgene00028859 | |
| Reference | WBPaper00026855 |
