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WormBase Tree Display for Construct: WBCnstr00011586

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Name Class

WBCnstr00011586Other_nameExpr3668_Ex
Summary[pie-1::gfp-C29E4.2]
Driven_by_geneWBGene00004027
GeneWBGene00016202
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[pie-1::gfp-C29E4.2] translational fusion. The sequence between the predicted initiation and termination codons, including the intervening introns, was amplified using genomic DNA as a template and primers containing appropriate restriction enzyme sites. These were subsequently cloned into GFP vectors driven by two different promoters: a pie-1 promoter vector, which drives expression of N-terminal GFP fusion protein in the germline (pie-1::GFP::in-ORF), and an npp-1 promoter vector, which drives expression of a C-terminal GFP fusion protein in the germline and the soma (npp-1::in-ORF::GFP).|[npp-1::C29E4.2-gfp] translational fusion.
Used_forTransgene_constructWBTransgene00028803
ReferenceWBPaper00026722