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WormBase Tree Display for Construct: WBCnstr00008391

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Name Class

WBCnstr00008391Public_namepEB73
Summary[Pmca-3::GFP]
Driven_by_geneWBGene00003153
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryClone = pEB73. This construct consists of a 2.5-kb region upstream of mca-3 ATG, amplified from genomic wild-type DNA with oligos oEB232 [AATTCTGCAGCACAATGGCTACAGTAGCC] and oEB237 [TTCTACCGGTACCCGAAGCTCCTCCAGTGATG]. These primers append PstI and AgeI restriction sites, respectively. The promoter fragment was then inserted into a PstI and AgeI digested Fire lab vector pPD95.75 to produce a transcriptional GFP fusion.
Used_forTransgene_constructWBTransgene00008688
ReferenceWBPaper00029172