WormBase Tree Display for Construct: WBCnstr00006286
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| WBCnstr00006286 | Summary | [Ptrp-4::caspase3-NZ, Pgrd-13::CZ-caspase3, Pelt-2::gfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006616 | ||
| WBGene00001692 | |||
| WBGene00001250 | |||
| Fusion_reporter | GFP | ||
| Other_reporter | caspase3-NZ | ||
| CZ-caspase3 | |||
| Construction_summary | This transgene kills the RnA and RnB neurons. The bxEx137 line was generated by co-injecting EM#317 and EM#318 plasmids (25 ng/l each) with the marker elt-2::gfp (50 ng/l) and RnA neuronal death was monitored by crossing bxEx137 into bxIs19. EM#317 (P(trp-4)::caspase3-NZ) was generated by removing the gfp coding region from EM#316 and introducing the caspase3-NZ coding region from the pTU813 plasmid (BamHI-EcoRI region) [10]. EM#318 (P(grd-13)::CZ- caspase3) was generated by inserting the 712 nt region upstream of the grd-13 gene (from 3911 to 4623 of W05E7) between the HindIII- BamHI sites of the CZ-caspase3 plasmid pTU#814 [10] (Barrios et al., 2008). | ||
| Used_for | Transgene_construct | WBTransgene00006439 | |
| Reference | WBPaper00032414 |
