WormBase Tree Display for Construct: WBCnstr00006004
expand all nodes | collapse all nodes | view schema
WBCnstr00006004 | Summary | [Ppie-1::gfp-sas-6(S123A)] | |
---|---|---|---|
Driven_by_gene | WBGene00004027 | ||
Gene | WBGene00012888 | ||
Fusion_reporter | GFP | ||
Construction_summary | For generating this transgenic line appropriate primers (sequences available upon request) were used to PCR-amplify sas-6 cDNA, replacing the AGT that normally codes serine 123 by GCT, and cloning the resulting fragments into pSU25 or pIC26, pie-1-based vectors containing a rescuing unc-119 cDNA (gifts from Michael Glotzer and Karen Oegema). (Kitagawa et al., 2010) | ||
Used_for | Transgene_construct | WBTransgene00006147 | |
Reference | WBPaper00035594 |