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WormBase Tree Display for Construct: WBCnstr00006004

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Name Class

WBCnstr00006004Summary[Ppie-1::gfp-sas-6(S123A)]
Driven_by_geneWBGene00004027
GeneWBGene00012888
Fusion_reporterGFP
Construction_summaryFor generating this transgenic line appropriate primers (sequences available upon request) were used to PCR-amplify sas-6 cDNA, replacing the AGT that normally codes serine 123 by GCT, and cloning the resulting fragments into pSU25 or pIC26, pie-1-based vectors containing a rescuing unc-119 cDNA (gifts from Michael Glotzer and Karen Oegema). (Kitagawa et al., 2010)
Used_forTransgene_constructWBTransgene00006147
ReferenceWBPaper00035594