WormBase Tree Display for Antibody: WBAntibody00000360
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| WBAntibody00000360 | Summary | Rabbit polyclonal antibody against recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc4641]:aip-1 | |||
| Gene | WBGene00000097 | |||
| Isolation | Location | SJ | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | A MYC (9E10) epitope-tagged AIRAP mammalian expression vector was constructed by overlapping PCR of AIRAP cDNA with oligos 5 GAC AGG ATC CAT AAT GGA GTT TCC TGA CT 3', 5'AGA GAT CAG CTT CTG CTC CTT CAC TGT GCG CCT GAG 3' and 5' GGG GCT CGA GTC ACA GAT CCT CCT CAG AGA TCA GCT TCT GCT C 3' and expressed from a pCDNA3 mammalian expression plasmid (Invitrogen). A bacterial expression plasmid encoding a full-length fusion of AIRAP and GST was constructed in pGEXKG (Pharmacia). The purified fusion protein was cleaved with thrombin, and the liberated intact AIRAP was used to immunize rabbits. | ||
| Animal | Rabbit | |||
| Reference | WBPaper00004641 |
