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WormBase Tree Display for Variation: WBVar02149950

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WBVar02149950	Evidence	Paper_evidence	WBPaper00029039
	Name	Public_name	kb26
	Sequence_details	SeqStatus	Pending_curation
	Variation_type	Allele
	Origin	Species	Caenorhabditis elegans
		Laboratory	VP
		Status	Live
	Affects	Gene	WBGene00000176
	Description	Phenotype_not_observed	WBPhenotype:0000039	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Data not shown.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
			WBPhenotype:0000145	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Data not shown.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
			WBPhenotype:0000518	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Data not shown.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
			WBPhenotype:0000643	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Data not shown.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
			WBPhenotype:0000673	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Data not shown.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
			WBPhenotype:0000876	Paper_evidence	WBPaper00029039
				Curator_confirmed	WBPerson557
				Remark	Authors monitored hypotonic stress resistance using mobility as a read out for resistance and saw no difference between mutant alleles and control worms while monitoring worm motility. Briefly, worms were grown on high-salt nematode growth medium for at least 1 week, L4 and young adult worms were switched to low-salt medium and mobility assayed.	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Variation_effect	Predicted_null_via_sequence	Paper_evidence	WBPaper00029039
						Curator_confirmed	WBPerson557
				Phenotype_assay	Treatment	Worms were grown on high-salt (365 mM NaCl) nematode growth medium (NGM) for at least 1 week. L4 and young adult worms were manually transferred from high-salt plates Low-salt (1 mM NaCl) NGM plates. Worms were manually transferred from high-salt plates to the side of low-salt plates lacking bacteria. The number of worms that reached the bacterial food source was counted every 10 min for the first 3 hours and every hour after that.	Paper_evidence	WBPaper00029039
							Curator_confirmed	WBPerson557
	Reference	WBPaper00029039
	Remark	Created in the nameserver by Karen Yook
		aqp-8 WBPaper00029039 1) All alleles contained deletions in one or more critical transmembrane helices and pore-lining NPA domains (Fig. 4A). Since water channel structure and function requires intact NPA and transmembrane domains (15), all deletion mutations are likely null alleles. 2) Deletion alleles were generated using a method described previously...this is ENU mutagenesis and thermostable restriction enzyme PCR screening.
	Method	Allele