WormBase Tree Display for Variation: WBVar00275520
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WBVar00275520 | Name | Public_name | zu193 | ||||||
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Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00007281 | ||||||||
WBStrain00007282 | |||||||||
Laboratory | JJ | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00003397 | |||||||
Interactor (14) | |||||||||
Description | Phenotype | WBPhenotype:0000047 | Paper_evidence | WBPaper00038341 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Cells internalize late at the 4E stage in 72% of embryos. | Paper_evidence | WBPaper00038341 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000050 | Paper_evidence | WBPaper00002871 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Complete | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000219 | Paper_evidence | WBPaper00031110 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals from strains from either the Sternberg lab or the Eisenmann lab exhibit an underinduction phenotype. | Paper_evidence | WBPaper00031110 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Probable_null_via_phenotype | Paper_evidence | WBPaper00031110 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000436 | Paper_evidence | WBPaper00026736 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | WRM-1 fails to be retained in the E nucleus | Paper_evidence | WBPaper00026736 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
EQ_annotations (2) | |||||||||
Phenotype_assay | Treatment | mom-5 mutants carrying the WRM-1::GFP transgene were examined. Nuclear retention was assayed by subjecting the animals to imb-4 RNAi | Paper_evidence | WBPaper00026736 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
Genotype | neIs2 [WRM-1::GFP] | Paper_evidence | WBPaper00026736 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0000472 | Paper_evidence | WBPaper00002871 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | Table 1 | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Low | 5% penetrance | Paper_evidence | WBPaper00002871 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000760 | Paper_evidence | WBPaper00003645 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | In mom-5(zu193) mutants, the EMS cell exhibited spindle orientation defects, with significant dorsal/ventral or left/right orientation components, in contrast to the normally anterior/posterior orientation of the wild type EMS spindle (data not shown). | Paper_evidence | WBPaper00003645 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
In EMS cells derived from isolated P1 blastomeres from mom-5(zu193) mutants, spindles exhibited orientation defects, with a wide range of orientation angles, showing nearly random orientations (Figure 2). | Paper_evidence | WBPaper00003645 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
In EMS blastomeres isolated from mom-5(zu193) mutant embryos and placed in contact with isolated wild type P2 blastomeres, EMS spindles exhibited orientation defects suggesting that mom-5 is required in EMS for proper EMS spindle orientation (Figure 4A). | Paper_evidence | WBPaper00003645 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | 57% penetrance | Paper_evidence | WBPaper00003645 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations (2) | |||||||||
Phenotype_assay | Treatment | Authors isolated the smaller, posterior-most blastomere in a two-cell-stage embryo, called P1, and allowed it to divide into its daughters, P2 and EMS. The orientation of the mitotic spindle in EMS was then measured relative to the plane of contact between EMS and P2. | Paper_evidence | WBPaper00003645 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
For genetically mosaic partial embryos, wild-type and mutant early EMS and P2 blastomeres were concurrently isolated. EMS and P2 were recombined early in the EMS cell cycle allowing the cells to be in contact for at least 9 min before EMS cytokinesis. | Paper_evidence | WBPaper00003645 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001495 | Paper_evidence | WBPaper00002871 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | E daughter cells of some (2/12) mom-5(zu193) mutant embryos divide earlier (compared to MS daughters) than in wild type embryos | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Incomplete | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006733 | PATO:0000460 | Paper_evidence | WBPaper00002871 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0006612 | PATO:0000460 | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001635 | Paper_evidence | WBPaper00002871 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | E blastomeres produced pharyngeal cells (aberrantly) instead of endoderm in 2/15 (13%) mom-5(zu193) mutants | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0004804 | PATO:0000460 | Paper_evidence | WBPaper00002871 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001637 | Paper_evidence | WBPaper00005375 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | 4% of mom-5(zu193) embryos lacked an intestine (Table 1) | Paper_evidence | WBPaper00005375 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Penetrance | Low | 4 | Paper_evidence | WBPaper00005375 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_not_observed | WBPhenotype:0000218 | Paper_evidence | WBPaper00031110 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals from strains from either the Sternberg lab or the Eisenmann lab do not exhibit a significant level of overinduction although a very low level (2%) of the animals from the Eisenmann strain exhibit an overinduced phenotype; 0% of the animals from the Sternberg strain exhibit OI. | Paper_evidence | WBPaper00031110 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Probable_null_via_phenotype | Paper_evidence | WBPaper00031110 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000760 | Paper_evidence | WBPaper00002871 | |||||||
WBPaper00003645 | |||||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | The ABar cells of pop-1(zu193) embryos exhibit normal spindle orientation, with the ABar spindle perpendicular to the ABpr spindle. | Paper_evidence | WBPaper00002871 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
In EMS blastomeres isolated from wild type embryos and placed in contact with isolated mom-5(zu193) mutant P2 blastomeres, EMS spindles exhibited wild type orientation suggesting that mom-5 is dispensable in P2 for proper EMS spindle orientation (Figure 4A). | Paper_evidence | WBPaper00003645 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations (2) | |||||||||
Phenotype_assay | Treatment | For genetically mosaic partial embryos, wild-type and mutant early EMS and P2 blastomeres were concurrently isolated. EMS and P2 were recombined early in the EMS cell cycle allowing the cells to be in contact for at least 9 min before EMS cytokinesis. | Paper_evidence | WBPaper00003645 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000883 | Paper_evidence | WBPaper00035405 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | Nerve ring development is normal | Paper_evidence | WBPaper00035405 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0001133 | Paper_evidence | WBPaper00005375 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | mom-5(zu193) animals exhibited no embryos with aberrant L-R division of EMS (Table 1) | Paper_evidence | WBPaper00005375 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006876 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001224 | Paper_evidence | WBPaper00060654 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | There are six Wnt receptors encoded in the C. elegans genome: four Frizzled receptors (LIN-17, CFZ-2, MIG-1 and MOM-5,), one Ror receptor (CAM-1) and one Ryk receptor (LIN-18) (Sawa and Korswagen, 2013). We analyzed the effect of loss-of-function mutations for each receptor and found that loss of cam-1, but not the other receptors, caused defective SMDD axonal development (Figure 1D). | Paper_evidence | WBPaper00060654 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0004972 | PATO:0000460 | Paper_evidence | WBPaper00060654 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0004971 | PATO:0000460 | Paper_evidence | WBPaper00060654 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00038341 | ||||||||
WBPaper00005375 | |||||||||
WBPaper00002871 | |||||||||
WBPaper00026736 | |||||||||
WBPaper00003645 | |||||||||
WBPaper00031110 | |||||||||
WBPaper00035405 | |||||||||
WBPaper00060654 | |||||||||
Method | Allele |