WormBase Tree Display for Variation: WBVar00275484

WBVar00275484 Evidence: Paper_evidence: WBPaper00001584
  Name: Public_name: zu120
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Laboratory: JJ
    Status: Live
  Affects: Gene: WBGene00003228
  Description: Phenotype: WBPhenotype:0000050 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000097 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: ABa and ABp blastomeres have abnormal lineages Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005994 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
            WBbt:0005906 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000414 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: P3-blastomere adopts a muscle fate (assayed via lineage analysis and antibody staining) Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
          ABa and ABp blastomeres adopt an MS-like fate. Additional blastomeres in 28-cell stage pie-1 embryos express the CeMyoD beta-galactosidase reporter. Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Penetrance: Complete: 100 percent Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0002474 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
            WBbt:0005906 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
            WBbt:0005994 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: P3 blastomeres are isolated via laser ablation, followed by staining with 5-6 antibody. Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
            Target blastomeres (such as ABa or ABp) are isolated via laser ablation, followed by staining with 3NB12. Embryos were also stained with LacZ Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000715 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: Mutant embryos produce excess muscle cells. Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: AB blastomeres were isolated via laser ablation, followed by staining with 5-6 monoclonal antibody (stains body wall-specific myosin). Embryos were also stained with LacZ Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001302 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: The restricted localization of P granules to germ cell precursors is altered. P granules remain dispersed throughout the Pl blastomere instead of becoming associated with the posterior-most cortex as in wild-type. During subsequent divisions, most of the P granules are localized correctly to the smaller daughters, but a substantial fraction remains in the larger daughters Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: P-granule specific antibody staining Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001635 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: Mutants have large clusters of excess pharyngeal cells. These clusters are surrounded by a basement membrane and some of them contain grinder-like structures Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Penetrance: Complete: 100 percent Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: antibody staining with 3NB12 or 9.2.1 (stain pharyngeal-specific muscle cells) Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001637 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: mex-1 alleles show a cold-sensitive defect in producing intestinal cells. Embryos that were lacking intestinal cells, contained larger numbers of hypodermal cells instead Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: 37 percent Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 16C, 25C Paper_evidence: WBPaper00001584
              Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0001642 Paper_evidence: WBPaper00001584
        Curator_confirmed: WBPerson2021
        Remark: Mutant embryos appear identical to wild-type embryos in terms of size, cleavage rate, and division axes up to the 28-cell stage (data not shown) Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00001584
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00001584
                Curator_confirmed: WBPerson2021
        Maternal: Strictly_maternal: Paper_evidence: WBPaper00001584
            Curator_confirmed: WBPerson2021
  Reference: WBPaper00001584
  Method: Allele