WormBase Tree Display for Variation: WBVar00275457
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| WBVar00275457 | Evidence | Paper_evidence | WBPaper00027723 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Public_name | zc32 | |||||||
| Sequence_details | SeqStatus | Pending_curation | |||||||
| Variation_type | Allele | ||||||||
| Origin | Species | Caenorhabditis elegans | |||||||
| Laboratory | SJ | ||||||||
| Status | Live | ||||||||
| Affects | Interactor (35) | ||||||||
| Description | Phenotype | WBPhenotype:0000154 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Larvae that hatched from mutant eggs placed at the nonpermissive temperature developed into slightly uncoordinated, hsp-60::gfp-active adults with a compromised germline and very few progeny (Figure 1C)" | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Genotype | zcIs9 [hsp-60::gfp] | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000643 | Paper_evidence | WBPaper00027723 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Larvae that hatched from mutant eggs placed at the nonpermissive temperature developed into slightly uncoordinated, hsp-60::gfp-active adults with a compromised germline and very few progeny (Figure 1C)" | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Genotype | zcIs9 [hsp-60::gfp] | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001236 | Paper_evidence | WBPaper00027723 | |||||||
| WBPaper00035985 | |||||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "A screen for temperature-sensitive (ts) mutations that conditionally activated the UPRmt yielded a line, zc32, which robustly activated hsp-60::gfp and hsp-6::gfp at the nonpermissive temperature (25°) and weakly activated the reporters at the permissive temperature (20°) (Figure 1B)." | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| "In two models of mitochondrial stress, feeding of spg-7(RNAi) as well as a previously characterized temperature-sensitive allele (zc32) (Benedetti et al., 2006), GFP fluorescence increased over the course of postembryonic development." | Paper_evidence | WBPaper00035985 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | GO_term | GO:0034514 | PATO:0000460 | Paper_evidence | WBPaper00027723 | ||||
| WBPaper00035985 | |||||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Genotype | zcIs9 [hsp-60::gfp] | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| zcIs13 [hsp-6::gfp] | Paper_evidence | WBPaper00027723 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| zcIs9 [hsp-60pr::GFP] | Paper_evidence | WBPaper00035985 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001372 | Paper_evidence | WBPaper00031084 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "We were unable to reliably detect the endogenous mito- chondrial chaperone promoters in complex with endoge- nous DVE-1 , even in stressed animals ; however , the endogenous protein was immunoprecipitated in a specific complex with the promoter of the hsp-60 pr : : gfp transgene ( the hsp-60 promoter is likely present at high copy number in the integrated array , increasing signal strength ) . Impor- tantly , the latter complex was increased by mitochondrial unfolded protein stress in mutant zc32 animals ( Figure 3E ) . These observations correlate activation of the UPR mt with binding of DVE-1 to mitochondrial chaperone genes ." | Paper_evidence | WBPaper00031084 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Genotype | hsp-60pr::gfp | Paper_evidence | WBPaper00031084 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0002434 | Paper_evidence | WBPaper00027723 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "At the nonpermissive temperature, zc32 mutant animals were selectively impaired in their ability to develop under RNAi that induces further mitochondrial stress, but their ability to cope with ER stress was comparable to the wild type (Figure 2)." | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | GO_term | GO:0034514 | PATO:0000460 | Paper_evidence | WBPaper00027723 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_not_observed | WBPhenotype:0001485 | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "At the nonpermissive temperature, zc32 mutant animals were selectively impaired in their ability to develop under RNAi that induces further mitochondrial stress, but their ability to cope with ER stress was comparable to the wild type (Figure 2)." | Paper_evidence | WBPaper00027723 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00027723 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Reference | WBPaper00027723 | ||||||||
| WBPaper00031084 | |||||||||
| WBPaper00035985 | |||||||||
| Method | Allele |
