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WormBase Tree Display for Variation: WBVar00249278

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Name Class

WBVar00249278EvidencePerson_evidenceWBPerson449
NamePublic_nametk27
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryNF
StatusLive
AffectsGeneWBGene00006876
InteractorWBInteraction000501345
WBInteraction000501346
DescriptionPhenotypeWBPhenotype:0000154Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
Remarktk27 hermaphrodites proliferated as homozygotes with small broods.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000188Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkMutants exhibited shortened gonad arms when compared with those in wild-type animals.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000195Paper_evidenceWBPaper00040080
Person_evidenceWBPerson449
Curator_confirmedWBPerson48
WBPerson712
RemarkThe migration rate of DTCs was globally slower and the DTCs were arrested after the second turn, compared to controls. | In vab-10(tk27) animals, the migration of DTCs occurred essentially in a similar pattern to that of wild type, although the lamellipodial extension was not as extensive.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000425Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkBoth anti-VAB-10N and anti-VAB-10B detected signals in wild-type DTCs, but they failed to do so in tk27 DTCs.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0006865PATO:0000460Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000511Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkThe nuclear translocation of DTCs at the dorsal turn was suppressed in vab-10(tk27) mutants. | Nuclei stayed at the anterior or posterior ends rather than being relocated to the dorsal side of the DTCs, as in the wild type.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000691Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkAnimals with this mutation exhibited defects in gonad development.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000692Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkMales were sterile (data not shown).Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0001588Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkIn the vab-10(tk27) mutant, the array of MT filaments was severely disorganized, and the polarized localization of MTBD-Venus was completely lost. | Randomized patterns of shooting comets in both front and rear regions in the vab-10(tk27) DTCs were observed.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000062Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkAnimals did not exhibit any lethality.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0000071Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkAnimals did not exhibit any head deformation.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
WBPhenotype:0001587Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
RemarkAlthough the F-actin tended to be oriented along the anteroposterior axis before the dorsal turn, they came together to form thick bundles that surrounded the edges of the concave cell surface at the trailing end of the DTCs at the initiation of the dorsal turn. This bundling was also observed for F-actin in vab-10(tk27) animals.Paper_evidenceWBPaper00040080
Curator_confirmedWBPerson712
ReferenceWBPaper00040080
MethodAllele