WormBase Tree Display for Variation: WBVar00241833
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| WBVar00241833 | Name | Public_name | s385 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Sequence_details | SeqStatus | Pending_curation | |||||||
| Variation_type | Allele | ||||||||
| Origin | Species | Caenorhabditis elegans | |||||||
| Strain | WBStrain00000645 | ||||||||
| Laboratory | BC | ||||||||
| Status | Live | ||||||||
| Affects | Gene | WBGene00003164 | |||||||
| Genetics | Mapping_data | In_pos_neg_data | 3431 | ||||||
| 3526 | |||||||||
| 3527 | |||||||||
| 3528 | |||||||||
| Description | Phenotype | WBPhenotype:0000056 | Paper_evidence | WBPaper00004853 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "We examined the let-425 homozygous animals laid by heterozygous mothers because homozygous animals are completely infertile. Two phenotypes were apparent in the let-425 homozygous progeny; late larval arrest and adult sterility, but they exhibited no embryonic lethality. In order to check if there was any embryonic lethality associated with the mutation, we examined the progeny from heterozygous mothers with the genotype dpy-18/dpy-18; unc-46 let-425/+. Among the 2145 progeny laid from the heterozygous mothers, we found that 25.7% were let-425 homozygous animals. This percentage is comparable with the expected number of homozygous let-425 progeny if there had not been any embryonic lethality associated with the let-425 mutation. In another experiment, we let the animals of the genotype dpy-18/dpy-18; unc-46 let-425/+ lay eggs and then transferred all the progeny individually to single plates and allowed them to grow. Among the 194 progeny examined, 25.8% were Dpy Unc larvae or sterile adults, while 47.9% were heterozygotes and 26.3% were dpy-18 homozygotes, confirming that there was no embryonic lethality and that the let-425 mutation caused 100% larval arrest or adult sterility." | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000059 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "...the let-425 mutant animals displayed hypo-induction of the vulval precursor cells, similar to animals affected by med-6 RNAi (Fig 2B,C) as well as larval arrest, a male tail defect and adult sterility. " | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000070 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "...the let-425 mutant animals displayed hypo-induction of the vulval precursor cells, similar to animals affected by med-6 RNAi (Fig 2B,C) as well as larval arrest, a male tail defect and adult sterility. " | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000186 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Next, we examined the phenotypes of the larvae and adults of the let-425 homozygous animals in more detail. Among the 132 animals observed under Nomarski optics, 72% were arrested in the L4 larval stage and 28% were adults with 100% sterility. The sterile adults were defective both in oogenesis and spermatogenesis: when the male progeny from RNAi-affected him-5 hermaphrodites were mated with unc-101 hermaphrodites, they could not produce any cross progeny (n=20). Additionally, in a reciprocal mating, in which RNA-affected hermaphrodites were mated with N2 males, no progeny were produced (data not shown). Because male tail defects and vulval defects, which may interfere with mating ability by themselves, are not 100% penetrant (see the following sections), enough numbers of matings should have resulted in production of cross progeny if there was no defect in spermatogenesis or oogenesis, respectively." | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000219 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "...the let-425 mutant animals displayed hypo-induction of the vulval precursor cells, similar to animals affected by med-6 RNAi (Fig 2B,C) as well as larval arrest, a male tail defect and adult sterility. " | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0007809 | PATO:0000460 | Paper_evidence | WBPaper00004853 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000670 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Next, we examined the phenotypes of the larvae and adults of the let-425 homozygous animals in more detail. Among the 132 animals observed under Nomarski optics, 72% were arrested in the L4 larval stage and 28% were adults with 100% sterility. The sterile adults were defective both in oogenesis and spermatogenesis: when the male progeny from RNAi-affected him-5 hermaphrodites were mated with unc-101 hermaphrodites, they could not produce any cross progeny (n=20). Additionally, in a reciprocal mating, in which RNA-affected hermaphrodites were mated with N2 males, no progeny were produced (data not shown). Because male tail defects and vulval defects, which may interfere with mating ability by themselves, are not 100% penetrant (see the following sections), enough numbers of matings should have resulted in production of cross progeny if there was no defect in spermatogenesis or oogenesis, respectively." | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000688 | Paper_evidence | WBPaper00001474 | |||||||
| WBPaper00004853 | |||||||||
| Person_evidence | WBPerson261 | ||||||||
| Curator_confirmed | WBPerson712 | ||||||||
| WBPerson2987 | |||||||||
| Remark | sterile adult | Person_evidence | WBPerson261 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| "...the let-425 mutant animals displayed hypo-induction of the vulval precursor cells, similar to animals affected by med-6 RNAi (Fig 2B,C) as well as larval arrest, a male tail defect and adult sterility. " | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000697 | Paper_evidence | WBPaper00004853 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Interestingly, the sterile adult animals showed an additional phenotype of protruding vulvae (Fig 2F)." | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_not_observed | WBPhenotype:0000050 | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "We examined the let-425 homozygous animals laid by heterozygous mothers because homozygous animals are completely infertile. Two phenotypes were apparent in the let-425 homozygous progeny; late larval arrest and adult sterility, but they exhibited no embryonic lethality. In order to check if there was any embryonic lethality associated with the mutation, we examined the progeny from heterozygous mothers with the genotype dpy-18/dpy-18; unc-46 let-425/+. Among the 2145 progeny laid from the heterozygous mothers, we found that 25.7% were let-425 homozygous animals. This percentage is comparable with the expected number of homozygous let-425 progeny if there had not been any embryonic lethality associated with the let-425 mutation. In another experiment, we let the animals of the genotype dpy-18/dpy-18; unc-46 let-425/+ lay eggs and then transferred all the progeny individually to single plates and allowed them to grow. Among the 194 progeny examined, 25.8% were Dpy Unc larvae or sterile adults, while 47.9% were heterozygotes and 26.3% were dpy-18 homozygotes, confirming that there was no embryonic lethality and that the let-425 mutation caused 100% larval arrest or adult sterility." | Paper_evidence | WBPaper00004853 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Reference | WBPaper00001474 | ||||||||
| WBPaper00004853 | |||||||||
| Method | Allele |
