WormBase Tree Display for Variation: WBVar00240985

WBVar00240985 Name: Public_name: q20
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004583
      WBStrain00022523
      WBStrain00022525
    Laboratory: JK
    Status: Live
  Affects: Gene: WBGene00001413
    Interactor: WBInteraction000050596
      WBInteraction000503024
      WBInteraction000524787
      WBInteraction000556052
      WBInteraction000556054
      WBInteraction000557074
      WBInteraction000557077
      WBInteraction000558239
  Genetics: Mapping_data: In_multi_point: 1911
  Description: Phenotype: WBPhenotype:0000038 Paper_evidence: WBPaper00050044
        Curator_confirmed: WBPerson17144
        Remark: Increase rupture post-reproductively Paper_evidence: WBPaper00050044
            Curator_confirmed: WBPerson17144
      WBPhenotype:0000416 Paper_evidence: WBPaper00039871
        Curator_confirmed: WBPerson712
        Remark: Yolk protein levels were elevated in animals. Paper_evidence: WBPaper00039871
            Curator_confirmed: WBPerson712
      WBPhenotype:0000683 Paper_evidence: WBPaper00028730
        Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: At 25C XX germline makes only sperm, at 15C XX germline makes oocytes and excess sperm. Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Semi_dominant: Person_evidence: WBPerson261
          Curator_confirmed: WBPerson712
        Variation_effect: Hypermorph_gain_of_function: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: 25 Person_evidence: WBPerson261
              Curator_confirmed: WBPerson712
        Ease_of_scoring: ES3_Easy_to_score: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0001779 Paper_evidence: WBPaper00035664
        Curator_confirmed: WBPerson2021
        Remark: miRNA 35-41 expression is disrupted Paper_evidence: WBPaper00035664
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00035664
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001823 Paper_evidence: WBPaper00028527
        Curator_confirmed: WBPerson2021
        Remark: Male sperm failed to accumulate in the spermatheca Paper_evidence: WBPaper00028527
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00028527
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Wild-type males labelled with MitoTracker were mated to non-labelled mutant hermaphrodites Paper_evidence: WBPaper00028527
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001824 Paper_evidence: WBPaper00035521
        Curator_confirmed: WBPerson712
        Remark: N2 prophase was completed between 16-21 hr; similarly, meiotic prophase kinetics of fem-3(gf) XX animals (25C) was completed by 21 hr. Paper_evidence: WBPaper00035521
            Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000113 Paper_evidence: WBPaper00031903
        Curator_confirmed: WBPerson712
        Remark: Expression of 21 U RNAs was normal. Paper_evidence: WBPaper00031903
            Curator_confirmed: WBPerson712
        Variation_effect: Hypermorph_gain_of_function: Paper_evidence: WBPaper00031903
            Curator_confirmed: WBPerson712
        EQ_annotations: Life_stage: WBls:0000038 PATO:0000460 Paper_evidence: WBPaper00031903
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Samples were enriched for RNAs <200 bp and used in northern analysis to examine the expression profile of the 21U-RNA 21ur-1 as Paper_evidence: WBPaper00031903
              Curator_confirmed: WBPerson712
      WBPhenotype:0000114 Paper_evidence: WBPaper00034714
          WBPaper00039871
        Curator_confirmed: WBPerson2021
          WBPerson712
        Remark: Expression of elt-1 was detectable during spermatogenesis in fem-3 adults beginning at pachytene and extending through the formation of haploid spermatids , the same pattern that was observed in wildtype L4 animals Paper_evidence: WBPaper00034714
            Curator_confirmed: WBPerson2021
          vit-2 and -5 mRNA levels were normal. The levels of sod-3 mRNA were also roughly normal. Paper_evidence: WBPaper00039871
            Curator_confirmed: WBPerson712
        Variation_effect: Hypermorph_gain_of_function: Paper_evidence: WBPaper00034714
              WBPaper00039871
            Curator_confirmed: WBPerson2021
              WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00034714
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: In situ hybridization with elt-1 antisense probe Paper_evidence: WBPaper00034714
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001182 Paper_evidence: WBPaper00032310
        Curator_confirmed: WBPerson2987
        Remark: fem-3(q20ts) mutants exhibited normal fat content at the nonpermissive temperature, compared to wild type controls, as determined by Nile Red staining (Figure 2A-D) Paper_evidence: WBPaper00032310
            Curator_confirmed: WBPerson2987
      WBPhenotype:0001410 Paper_evidence: WBPaper00040337
        Curator_confirmed: WBPerson712
        Remark: Antibodies that recognize the nearly identical GSP-3 and GSP-4 (GSP-3/4) proteins were used to show that, similar to the sperm-specific MSP protein, GSP-3/4 are expressed only in sperm-producing fem-3(q20gf) but not oocyte-producing fem-1(hc17lf) mutant hermaphrodites. Paper_evidence: WBPaper00040337
            Curator_confirmed: WBPerson712
  Reference (17)
  Method: Allele