WormBase Tree Display for Variation: WBVar00239177

WBVar00239177 Name: Public_name: pk35
    Other_name: pk35te
      E02C12.5a.1:c.118+272_961del
    HGVSg: CHROMOSOME_V:g.9357839_9359361del
  Sequence_details: SMap: S_parent: Sequence: E02C12
    Flanking_sequences: ccttctgttctcgaacactttttcaaaaaa tgcatcagacgtgtgcaacagatacagatc
    Mapping_target: E02C12
    Type_of_mutation: Insertion
      Deletion
    SeqStatus: Sequenced
  Variation_type: Allele
    Transposon_insertion: Tc1
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00007863
      WBStrain00028900
      WBStrain00028902
      WBStrain00028996
    Laboratory: NL
    Author: Zwaal RR
      Plasterk RHA
    Status: Live
  Affects: Gene: WBGene00001665
    Transcript: E02C12.5a.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: E02C12.5a.1:c.118+272_961del
        cDNA_position: ?-985
        CDS_position: ?-961
        Protein_position: ?-321
        Intron_number: 2-6/8
        Exon_number: 3-7/9
    Interactor: WBInteraction000501261
      WBInteraction000501273
      WBInteraction000521352
      WBInteraction000523953
  Isolation: Mutagen: Tc1
    Reverse_genetics: Derived from Tc1 insertion allele pk12
    Derived_from_variation: WBVar00239154
  Genetics: Interpolated_map_position: V 2.01507
  Description: Phenotype (21)
    Phenotype_not_observed: WBPhenotype:0000061 Paper_evidence: WBPaper00033456
        Curator_confirmed: WBPerson2021
        Remark: Life span is unaffected in gpa-3(pk35) mutants Paper_evidence: WBPaper00033456
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00033456
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000114 Paper_evidence: WBPaper00033456
        Curator_confirmed: WBPerson2021
        Remark: sod-3 mRNA level in gpa-3(pk35) remained unchanged relative to N2 wild type. No change in mRNA levels of PDE-1 and PDE-5 in gpa-3(pk35) mutants Paper_evidence: WBPaper00033456
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00033456
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000679 Paper_evidence: WBPaper00048972
        Curator_confirmed: WBPerson2987
        Remark: "Since the animals harboring the dominant active version of GPA-3 showed a slight decrease in fluorescence intensity of GFP::RAB-5, we wondered whether the opposite happens in the gpa-3(pk35) loss-of-function mutant. However, gpa-3(pk35) animals showed wild type fluorescence intensity levels (Fig 6A and 6D and 6E)." Paper_evidence: WBPaper00048972
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: GFP::RAB-5 Paper_evidence: WBPaper00048972
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001414 Paper_evidence: WBPaper00003465
        Curator_confirmed: WBPerson291
      WBPhenotype:0001700 Paper_evidence: WBPaper00051284
        Curator_confirmed: WBPerson37651
        Remark: Figure 6C Paper_evidence: WBPaper00051284
            Curator_confirmed: WBPerson37651
        Phenotype_assay: Control_strain: WBStrain00000001 Paper_evidence: WBPaper00051284
              Curator_confirmed: WBPerson37651
      WBPhenotype:0001765 Paper_evidence: WBPaper00031936
        Curator_confirmed: WBPerson2021
        Remark: Animals with mutations in individual G protein subunits respond normally to CO2 Paper_evidence: WBPaper00031936
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00031936
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 10% CO2 Paper_evidence: WBPaper00031936
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001818 Paper_evidence: WBPaper00035254
        Curator_confirmed: WBPerson2021
        Remark: Calcium dynamics in adult ASK neurons in response to C3 and C6 were unaffected in gpa-3 mutant animals Paper_evidence: WBPaper00035254
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035254
            Curator_confirmed: WBPerson2021
      WBPhenotype:0002055 Paper_evidence: WBPaper00036207
        Curator_confirmed: WBPerson712
        Remark: Photocurrents appeared to be normal. Paper_evidence: WBPaper00036207
            Curator_confirmed: WBPerson712
      WBPhenotype:0002212 Paper_evidence: WBPaper00003465
        Curator_confirmed: WBPerson291
  Reference: WBPaper00001784
    WBPaper00043908
    WBPaper00031936
    WBPaper00033456
    WBPaper00003465
    WBPaper00036207
    WBPaper00035254
    WBPaper00048972
    WBPaper00051284
  Remark: Insertion is a tc1 element
  Method: Transposon_insertion