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WormBase Tree Display for Variation: WBVar00144590

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Name Class

WBVar00144590EvidencePaper_evidenceWBPaper00001677
WBPaper00038033
NamePublic_namee2141
Other_nameF02A9.6.1:c.2920C>T
CE00237:p.Arg974Cys
HGVSgCHROMOSOME_III:g.9098313C>T
Sequence_detailsSMapS_parentSequenceF02A9
Flanking_sequencesactgctctgatgctagctgttcgtgcacacgtgtcagactttccgtagtgcttcttcgtg
Mapping_targetF02A9
Type_of_mutationSubstitutionctPaper_evidenceWBPaper00038033
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (50)
LaboratoryCB
AA
SHU
StatusLive
AffectsGeneWBGene00001609
TranscriptF02A9.6.1 (12)
Interactor (74)
IsolationMutagenEMS
GeneticsInterpolated_map_positionIII0.163959
Mapping_dataIn_multi_point1508
DescriptionPhenotype (21)
Phenotype_not_observedWBPhenotype:0000207Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-1(e2141ts) mutants exhibited normal defectation rates at the non-permissive temperature of 25 degrees Celsius, compared to wild type controls (Figure 1J)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentFor defecation rate, 2-day-old well-fed adults after temperature shift at L4 larval stage were monitored. For each animal, the duration of two consecutive cycles was recorded 5 times and the average of ~5-10 animals is presented for each genotype.Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Temperature25Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0001006Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-1(e2141ts) mutants exhibited normal pharyngeal pumping rates at the non-permissive temperature of 25 degrees Celsius, compared to wild type controls (Figure 1H)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentFor pharyngeal pumping rate, 2-day-old well-fed adults after temperature shift at L4 larval stage were recorded with a video camera for 5 minutes. The number of pharyngeal contractions in each second interval was calculated. For each genotype, the average of ~15-20 animals was noted.Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Temperature25Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0001182Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-1(e2141ts) mutants exhibited normal fat content at the permissive temperature of 20 degrees Celsius, compared to wild type controls, as determined by Nile Red staining (Figure S3A,B)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTemperature20Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0001266Paper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
RemarkNematodes containing a mutation in either glp-1 or glp-4 did not have substantially more phospho-PMK-1 than did wild-type nematodesPaper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
WBPhenotype:0001381Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
Remarkglp-1(e2141) mutant worms were not resistant to anoxia exposure when fed a glucose-supplemented diet (Figure S1B)Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003916Paper_evidenceWBPaper00046527
Curator_confirmedWBPerson2987
WBPhenotype:0001800Paper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
Remarkglp-1 mutation did not significantly alter expression of four putative antimicrobial genes (lys-8, clec-85, dod-22, and K08D8.5) when the nematodes were exposed to pathogenic bacteriumPaper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
WBPhenotype:0002271Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-1(e2141ts) mutants exhibited normal food absorption rates at the non-permissive temperature of 25 degrees Celsius, compared to wild type controls (Figure 1I)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentFood absorption rate were measured as in O'Rourke et al. (in preparation). Briefly, 2-day old animals were placed onto C1-BODIPY-C12-containing NGM plates. After a 6 hour incubation in darkness, fluorescence images were captured every hour for 4 hours. C1-BODIPY-C12 staining fluorescence was quantified. The accumulation rate of C1-BODIPY-C12 overtime was calculated for both wild type and glp-1 mutants. The values of the mutants were then normalized to wild type. For each genotype at each time point, the average of 5 animals was noted.Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Temperature25Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0004025Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-1(e2141ts) mutants exhibited normal mean velocity at the non-permissive temperature of 25 degrees Celsius, compared to wild type controls (Figure 1K)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTreatment10 3-day-old well-fed adults after temperature shift at L4 larval stage were placed onto a fresh NGM plate seeded with 50 microliters of OP50 bacteria. 5 minutes Digital videos were captured using Zeiss Discovery V.12 stereoscope, Zeiss AxioCam Hsc camera and AxioVision software. Each individual was followed by the "worm tracking" function with the AxioVision software to quantify velocity. For each genotype, 3 plates were prepared to take videos and 20 individuals' activities were analyzed.Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Temperature25Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Reference (23)
RemarkIn addition to the curated substitution, e2141 also has an a to g substitution with flanking sequences atcaaaagagcaggatctcgaaaaacgcca & caagtgcagcatcgtctcgcgaaacaaatcPaper_evidenceWBPaper00038033
Contrary to what was previously thought and published (Kodoyianni et al., 1992 and also in WormBase), e2141 and e2144 do not bear the same sequence change. e2141 actually carries two mutations in exon 8: c2920t and a3610g.
MethodSubstitution_allele