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WormBase Tree Display for Variation: WBVar00144287

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Name Class

WBVar00144287EvidencePaper_evidenceWBPaper00002345
NamePublic_namee1763
Sequence_detailsSMapS_parentSequenceCHROMOSOME_X
Flanking_sequencesgaacactatctcctctccattgtctattttttttcactcccggcgatttgccgatttgct
Mapping_targetCHROMOSOME_X
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00030840
LaboratoryCB
StatusLive
AffectsGeneWBGene00023498
WBGene00023497
TranscriptZK678.1.1
ZK662.4.1
ZK662.4.2
InteractorWBInteraction000000671
WBInteraction000500666
WBInteraction000500676
WBInteraction000504229
WBInteraction000505213
WBInteraction000524137
WBInteraction000524143
WBInteraction000524144
GeneticsInterpolated_map_positionX22.9456
DescriptionPhenotypeWBPhenotype:0000216Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Remarkdefect in Bδ cell fate specificationPaper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0008451PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0001708PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0000414Paper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
Remarklin-15(e1763) resulted in a 54% penetrant P11 to P12 cell fate transformation (Table 1A)Paper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
PenetranceIncomplete54% penetrancePaper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004410PATO:0000460Paper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
WBbt:0004409PATO:0000460Paper_evidenceWBPaper00003135
Curator_confirmedWBPerson2987
WBPhenotype:0000425Paper_evidenceWBPaper00037906
Curator_confirmedWBPerson712
RemarkLIN-15A nuclear staining is greatly reduced in these mutants.Paper_evidenceWBPaper00037906
Curator_confirmedWBPerson712
WBPhenotype:0000700Paper_evidenceWBPaper00000762
WBPaper00037906
Curator_confirmedWBPerson2021
WBPerson712
Remark2 or 3 large ventral protrusionsPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
This phenotype is not rescued by overexpression of lin-56.Paper_evidenceWBPaper00037906
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
WBls:0000056PATO:0000460Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Phenotype_assayGenotypehim-5(e1467)Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
WBPhenotype:0001276Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
RemarksyIs45 expressed in Bδ (abnormal spicule development)Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0008451PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0010467PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0001487Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
RemarksyIs45 expressed in Bδ (abnormal spicule development)Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0008451PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0048608PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_not_observedWBPhenotype:0000134Paper_evidenceWBPaper00037906
Curator_confirmedWBPerson712
RemarkLIN-15A RNA levels are not reduced in these mutants compared to wild type, as measured through RT-PCR.Paper_evidenceWBPaper00037906
Curator_confirmedWBPerson712
WBPhenotype:0000961Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Remark"In a lin-15 loss-of-function background, all VPCs often adopt vulval fates, and P6.p is always the closest VPC to the AC and adopts the 1&deg; fate. We observed wild-type zmp-1::GFP expression in 39 of 41 P6.p lineages (Table 5; the remaining two animals had an abnormal gonad). Therefore, lin-15 is not required for normal 1&deg; patterning. Furthermore, unlike let-23(gf), the lin-15(lf) mutation does not suppress the 1&deg; patterning defect caused by the absence of the AC. When the AC was ablated at the late P6.px stage or the early P6.pxx stage, the expression patterns of zmp-1::GFP in P6.pxxx cells were disturbed in 17 of 19 animals examined (P<0.0001, Table 5). Thus, lin-15 is likely not involved in negatively regulating RAS signaling during 1&deg; lineage patterning."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs49 [zmp-1::GFP]Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Reference (11)
MethodDeletion_allele