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WormBase Tree Display for Variation: WBVar00144036

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Name Class

WBVar00144036NamePublic_namee1487
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (21)
LaboratoryCB
StatusLive
AffectsGeneWBGene00008140
GeneticsMapping_dataIn_multi_point55
DescriptionPhenotypeWBPhenotype:0000050Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
Remark15.9% eggs never hatch, yet are refractile; wild type animals produce 0.8% inviable zygotes.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals have an average brood size of 20924 s.d. (6 broods counted), wild type has average brood size 33034 s.d. (8 broods counted).Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000863Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkMales exhibit 89.1% wild type fertility.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001175Paper_evidenceWBPaper00000565
WBPaper00000179
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkMale self-progeny make up 4.2% of the population as measured from 8 broods, 1497 total animals.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Animals segregate 4.5% males compared to 0.3% segregated by wild type animals.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
self-progeny 5% malesPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Ease_of_scoringES3_Easy_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001583Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals segregate a small percentage (1.8%) of short animals shown to be 3X hermaphrodites (wild type segregates 0.04% 3X herm.).Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001631Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remark2% nullo-X ova.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000143Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000276Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentX-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000517Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals are indistinguishable from wild type in terms of behavior.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000520Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkBoth hermaphrodites and males are indistinguishable from wild type in terms of superficial anatomy.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001171Paper_evidenceWBPaper00044163
Curator_confirmedWBPerson1684
WBPhenotype:0001631Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkThe sex ratio of cross progeny sired by him males does not significantly differ from 1:1.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
ReferenceWBPaper00000565
WBPaper00000179
WBPaper00044163
WBPaper00060301
Remark[210303 skd] Primers and PCR conditions for genotyping are provided in Table S5 in WBPaper00060301Paper_evidenceWBPaper00060301
Curator_confirmedWBPerson51134
MethodAllele