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WormBase Tree Display for Variation: WBVar00143562

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Name Class

WBVar00143562EvidencePaper_evidenceWBPaper00005919
NamePublic_namee879
Sequence_detailsMapping_targetF28B3
Type_of_mutationSubstitutionct
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (12)
LaboratoryCB
StatusLive
AffectsGeneWBGene00001860
GeneticsMapping_dataIn_2_point259
In_multi_point9
10
11
998
1690
1897
3117
In_pos_neg_data6356
DescriptionPhenotypeWBPhenotype:0000050Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
Remark5.8% eggs never hatch, yet are refractile; wild type animals produce 0.8% inviable zygotes.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000143Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals have an average brood size of 20934 s.d. (11 broods counted), wild type has average brood size 33034 s.d. (8 broods counted).Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000624Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkA proper connection is never formed between the testis and the cloaca in males, in addition, the testis develops in a disorganized manner as determined by Nomarski and Feulgen staining of live animals.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005062PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000742Paper_evidenceWBPaper00000179
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkAnimals exhibit reduced recombination (58%) compared to wild type.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
X chromosome recombination specifically reduced 40% or more, except at left end (recombination increased).Person_evidenceWBPerson261
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000863Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkMales exhibit 1.4% wild type fertility.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001175Paper_evidenceWBPaper00000565
WBPaper00000179
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkMale self-progeny make up 29.2% population as measured from 16 broods, 1454 total animals.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Animals segregate 20.6% males compared to 0.3% segregated by wild type animals.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
Self-progeny 21% XO male, 5% 3X herm; 8% ova are nullo-X as a result of reductional meiotic nondisjunction. e879/+ self-progeny 1% XO.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Semi_dominantPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Ease_of_scoringES3_Easy_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001583Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals segregate a small percentage (5.6%) of short animals shown to be 3X hermaphrodites (wild type segregates 0.04% 3X herm.).Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001664Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkSelf-progeny 21% XO male, 5% 3X herm; 8% ova are nullo-X as a result of reductional meiotic nondisjunction; e879/+ self-progeny 1% XO.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001666Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals have extreme sensitivity to X-ray radiation.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentX-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000517Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAnimals are indistinguishable from wild type in terms of behavior.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000520Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkBoth hermaphrodites and males are indistinguishable from wild type in terms of superficial anatomy.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000648Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkMating behavior seemed normal.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0000670Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkAs determined by Nomarski and Feulgen staining of live animals.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
WBPhenotype:0001631Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
RemarkThe sex ratio of cross progeny sired by him males does not significantly differ from 1:1.Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000179
Curator_confirmedWBPerson712
ReferenceWBPaper00016553
WBPaper00000565
WBPaper00016084
WBPaper00000179
WBPaper00016397
WBPaper00014150
WBPaper00065293
MethodSubstitution_allele