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WormBase Tree Display for Variation: WBVar00143087

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Name Class

WBVar00143087NamePublic_namee286
Other_nameF30H5.1.1:c.2464C>T
CE01925:p.Leu822Phe
HGVSgCHROMOSOME_III:g.500483C>T
Sequence_details (5)
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00004142
WBStrain00006360
WBStrain00026328
WBStrain00033497
WBStrain00033521
WBStrain00034257
WBStrain00035413
LaboratoryCB
StatusLive
AffectsGeneWBGene00006781
TranscriptF30H5.1.1 (12)
InteractorWBInteraction000534891
WBInteraction000534893
WBInteraction000534895
WBInteraction000534897
WBInteraction000560388
IsolationMutagenhydroxylamine
GeneticsInterpolated_map_positionIII-26.82
Mapping_dataIn_2_point50
623
6052
In_multi_point67
856
857
1580
1628
1630
1632
2337
2338
3291
DescriptionPhenotypeWBPhenotype:0000306Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
RemarkUCS accumulation was significantly lower than that of the other transgenic products, TPR(-) UCS(-), and full-length UNC-45.Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
WBPhenotype:0000349Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkat 25C limpPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000509Paper_evidenceWBPaper00000536
Curator_confirmedWBPerson712
RemarkOnly 3.4% of sperm have pseudopods, some of these cells have filaments.Paper_evidenceWBPaper00000536
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0006798PATO:0000460Paper_evidenceWBPaper00000536
Curator_confirmedWBPerson712
WBPhenotype:0000640Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkat 25C EglPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00046852
Person_evidenceWBPerson2251
Curator_confirmedWBPerson2021
WBPerson2987
Remark"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0000644Paper_evidenceWBPaper00044758
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPerson5063
RemarkAt 25C paralyzed; males move better; at 20C slow, at 15C wildtype.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
temperature sensitive phenotype rescued by FUdRPaper_evidenceWBPaper00044758
Curator_confirmedWBPerson5063
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00044758
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPerson5063
Ease_of_scoringES3_Easy_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000646Paper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
WBPhenotype:0000782Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkfew thick filamentsPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000861Paper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
Remarkdefect in body muscle cellsPaper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
WBPhenotype:0000926Paper_evidenceWBPaper00000031
Person_evidenceWBPerson261
Curator_confirmedWBPerson48
WBPerson712
Remarkdefect in body muscle cellsPaper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
muscle ultrastructure defectivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001213Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
RemarkFull length UNC-45, TPR(-)UNC-45 and UCS(only) UNC-45, but not the UCS(-)UNC-45 construct were able to rescue the motility defect of e286 at the 25 deg C restrictive temperature.Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
WBPhenotype:0001292Paper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
Remarkdefect in body muscle cellsPaper_evidenceWBPaper00000031
Curator_confirmedWBPerson48
WBPhenotype:0001403Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"Animals expressing unc-45(e286) or unc-45(m94) that were shifted to restrictive conditions after the first larval stage exhibited disruption of myofilament structure associated with mislocalization of MYO-3, while wild-type animals were unaffected (Figure 3B)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature25Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0001414Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001569Paper_evidenceWBPaper00040187
WBPaper00046852
Curator_confirmedWBPerson712
WBPerson2987
Remarke286 worms grown at 25 deg C had abnormal thick filament assembly with no detectable myosin organization. A-band assembly was rescued in transgenic full length, TPR(-) and UCS(only) animals, but not in the UCS(-) transgenic animal.Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00040187
Curator_confirmedWBPerson712
WBPhenotype:0001889Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"Animals expressing unc-45(e286) or unc-45(m94) that were shifted to restrictive conditions after the first larval stage exhibited disruption of myofilament structure associated with mislocalization of MYO-3, while wild-type animals were unaffected (Figure 3B)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature25Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000518Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Remark"A single amino acid substitute in the UCS domain of C-elegans UNC-45, E781K, or L822F (the m94 and e286 alleles, respectively), results in temperature-sensitive motility defects (Figure 1B) and myosin disorganization phenotypes (Figure 1C) when animals are grown at restrictive conditions (>22 degrees Celsius). However, animals show no phenotypes during development and early adulthood when grown at the permissive temperature (15 degrees Celsius C)."Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
Phenotype_assayTemperature15Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson2987
WBPhenotype:0000644Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
RemarkAt 15C animals are WT on control RNAi but are fully paralyzed on daf-21, C01G10.8, sti-1 or ZC395.10 RNAi. WT animals do not show paralysis when treated with these RNAi.Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
Phenotype_assayTemperature15Paper_evidenceWBPaper00046852
Curator_confirmedWBPerson5063
WBPhenotype:0001645Paper_evidenceWBPaper00040284
Curator_confirmedWBPerson2987
RemarkTable S2Paper_evidenceWBPaper00040284
Curator_confirmedWBPerson2987
Reference (12)
MethodSubstitution_allele