WormBase Tree Display for Variation: WBVar00091750

WBVar00091750 Evidence: Paper_evidence: WBPaper00006537
  Name: Public_name: ok459
    HGVSg: CHROMOSOME_I:g.6183355_6184776del
  Sequence_details: SMap: S_parent: Sequence: T09B4
    Flanking_sequences: ctggagcgttctgaaaccacaattccgttt cataatattttatgtttaaataacttttta
    Mapping_target: T09B4
    Type_of_mutation: Deletion
    PCR_product: ok459_external
      ok459_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00035717
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00000500
      WBGene00020376
    Transcript: T09B4.2.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        Intron_number: 2-3/5
        Exon_number: 1-3/6
      T09B4.10.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        Intron_number: 2-5/8
        Exon_number: 1-5/9
  Isolation: Mutagen: UV/TMP
  Genetics: Mapping_data: In_multi_point: 4217
  Description: Phenotype: WBPhenotype:0000062 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: 25% (n=120) of the heat shocked CHIP (-/-)nematode larvae were dead (no movement at all). On the other hand, 100% (n=530) of heat shocked control animals (wild type) were alive (contract pharynx and thrash body). 4.9% (n=121) of the CHIP (-/-) larvae grown at 20 C (optimum growth temperature) were also dead (no movement at all). Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00006537
              Curator_confirmed: WBPerson712
      WBPhenotype:0000081 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: The homozygous ok459 larvae arrested at L1 stage. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
      WBPhenotype:0000273 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: Body thrashing rates of mutants are very low compared to wild type animals. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
      WBPhenotype:0000641 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: ok459 animals were either lethargic or unable to move. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
      WBPhenotype:0000747 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: ok459 animals were completely unable to contract pharynx. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
      WBPhenotype:0001482 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: ok459 animals bend their body occasionally. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
      WBPhenotype:0001983 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: ok459 animals do not exhibit exploratory behavior. Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000709 Paper_evidence: WBPaper00006537
        Curator_confirmed: WBPerson712
        Remark: The gross morphology of the pharynx of ok459 animals was seen like wild type animal (data not shown). Paper_evidence: WBPaper00006537
            Curator_confirmed: WBPerson712
  Reference: WBPaper00006537
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele