WormBase Tree Display for Variation: WBVar00089524

WBVar00089524 Evidence: Paper_evidence: WBPaper00005774
  Name: Public_name: n477
  Sequence_details: SMap: S_parent: Sequence: F55A8
    Flanking_sequences: gtcgacgacttccgagaggagtttgcacag ctgaaaaatgtgaagaggctagcgacactt
    Mapping_target: F55A8
    Type_of_mutation: Insertion
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00026778
    Laboratory: MT
    Status: Live
  Affects: Gene: WBGene00001173
    Transcript: F55A8.2g.1
      F55A8.2d.1
      F55A8.2e.1
      F55A8.2f.1
      F55A8.2b.1
      F55A8.2c.1
      F55A8.2a.2
      F55A8.2h.1
      F55A8.2a.1
    Interactor: WBInteraction000500289
  Genetics: Interpolated_map_position: IV -14.2686
  Description: Phenotype: WBPhenotype:0000006 Paper_evidence: WBPaper00000635
        Curator_confirmed: WBPerson48
      WBPhenotype:0000022 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have increased body length Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0000130 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: All alleles of egl-4 exhibit hypersensitivity to dauer pheromone Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 25C Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000227 Paper_evidence: WBPaper00061691
        Curator_confirmed: WBPerson712
        Remark: "...egl-4 loss-of-function mutant males were defective in their turning behavior and frequently became detached from the hermaphrodite while attempting turns. Quantification of this defect revealed that, compared to those performed by wild-type males, many more of the attempted turns made by egl-4 mutant males were aberrant." Paper_evidence: WBPaper00061691
            Curator_confirmed: WBPerson712
      WBPhenotype:0000294 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have dark intestines (Din) Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0000303 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants fail to respond to all concentrations of diacetyl tested Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00002819 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000304 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 alleles exhibit weaker but significant defects in the response to isoamyl alcohol Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00001063 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000480 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 alleles exhibit weaker but significant defects in the response to pyrazine Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00005360 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000545 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants retain approximately three times as many eggs as wild-type adults Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00004310
                Curator_confirmed: WBPerson2021
      WBPhenotype:0000639 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 alleles exhibit a Daf-c phenotype to varying degrees at 27C Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Phenotype_assay: Temperature: 27C Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000662 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: Mutants have foraging defects (data not shown) Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0001084 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have strong defects in the response to NaCl Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00003571 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001085 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 alleles exhibit weaker but significant defects in the response to butanone Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00005086 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001414 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: Mutants have male mating defects (data not shown) Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0001483 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants lay eggs at a later developmental stage, likely as a consequence of delayed active egg-laying periods Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00004310
                Curator_confirmed: WBPerson2021
      WBPhenotype:0001500 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have strong defects in the response to 2,3-pentanedione Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00001715 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001524 Paper_evidence: WBPaper00035944
        Curator_confirmed: WBPerson2776
          WBPerson10038
        Remark: Quoted from paper: "However egl-4 mutants still went into quiescence albeit for a shorter duration in 0.01 mM aldicarb. Taken together these data suggest that increased quiescent bout durations in liquid due to increased cholinergic activity require egl-4 function." Paper_evidence: WBPaper00035944
            Curator_confirmed: WBPerson10038
      WBPhenotype:0001565 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have strong defects in the response to lysine Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0001624 Paper_evidence: WBPaper00061691
        Curator_confirmed: WBPerson712
        Remark: "...induction of tail curling by exogenous serotonin was less efficient in egl-4 mutant males than in wild type." Paper_evidence: WBPaper00061691
            Curator_confirmed: WBPerson712
      WBPhenotype:0002114 Paper_evidence: WBPaper00035944
        Curator_confirmed: WBPerson2776
    Phenotype_not_observed: WBPhenotype:0000249 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants were found to be wild type in their responses to osmotic shock (data not shown). Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0000302 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants have normal responses to the odorant benzaldehyde Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00002087 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000398 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants were found to be wild type in their responses to gentle body touch (data not shown). Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0000660 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants exhibit little if any clumpy behavior (iwhere animals tend to congregate in clumps) Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0001086 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: All egl-4 mutants except n478 exhibit wild-type response to trimethylthiazole Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00004538 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001221 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants were found to be wild type in their responses to nose touch (data not shown). Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0001448 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants were found to be wild type in their responses to volatile repellents (data not shown). Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0002535 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: egl-4 mutants dye-fill normally and no obvious defects in the morphology of the neurons were visible (data not shown). Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00004310
          Curator_confirmed: WBPerson2021
      WBPhenotype:0004030 Paper_evidence: WBPaper00061691
        Curator_confirmed: WBPerson712
        Remark: "egl-4 loss-of-function mutant males tended to respond slightly less efficiently than wild-type males in standard response assays...but in the presence of a large excess of hermaphrodites almost all egl-4 mutant males were able to respond" Paper_evidence: WBPaper00061691
            Curator_confirmed: WBPerson712
  Reference: WBPaper00004310
    WBPaper00010745
    WBPaper00000635
    WBPaper00017625
    WBPaper00010687
    WBPaper00011220
    WBPaper00023070
    WBPaper00035944
    WBPaper00018461
    WBPaper00061691
  Remark: Flanking sequences are based on 30 bp to the left and right of V464 and T465 Curator_confirmed: WBPerson1845
    n477 has an eight-base pair insertion between V464 and T465, which causes a frameshift and a stop codon and leads to loss of the kinase domain Paper_evidence: WBPaper00005774
  Method: Insertion_allele