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WormBase Tree Display for Variation: WBVar00089078

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Name Class

WBVar00089078EvidencePaper_evidenceWBPaper00000565
NamePublic_namemn162
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00034253
LaboratorySP
StatusLive
AffectsGeneWBGene00004295
DescriptionPhenotypeWBPhenotype:0000032Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals produce large numbers of sick progeny (16.0% versus 0.4% for wild type).Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000050Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals exhibit a large increase in the number of inviable zygotes (62.2%) versus wild type (0.2%).Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkBrood sizes were reduced by factors of greater than 10. Brood sizes were further significantly reduced at 25C.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Reduced brood size (9% of wildtype)Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTemperature20CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Ease_of_scoringES1_Very_hard_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000275Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkAnimals exhibit moderate levels of UV hypersensitivity versus extreme hypersensitivity.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
hypersensitive to UVPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000784Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMale fertility was 1% that of wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentNOTE: males were generated by addition of him-5 to the strain as repeated attempts to generate males by heat-shock were unsuccessful.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Genotypehim-5(e1467)Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000276Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknot hypersensitive to X irradiationPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentX-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000392Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000498Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknot hypersensitive to MMSPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000520Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals were indistinguishable in appearance from wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001175Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001631Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMales produced equal numbers of X-bearing and nullo-X-bearing sperm.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayGenotypehim-5(e1467)Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
ReferenceWBPaper00000565
MethodAllele