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WormBase Tree Display for Variation: WBVar00089077

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Name Class

WBVar00089077EvidencePaper_evidenceWBPaper00000565
NamePublic_namemn161
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00034252
LaboratorySP
StatusLive
AffectsGeneWBGene00004293
GeneticsMapping_dataIn_multi_point371
DescriptionPhenotypeWBPhenotype:0000050Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals exhibit an increase in the number of inviable zygotes (3.2%) versus wild type (0.2%).Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
PenetranceLowPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkBrood sizes were further significantly reduced at 25C.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Reduced brood size (25% of wildtype)Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTemperature20CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000275Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkAnimals exhibit moderate levels of UV hypersensitivity versus extreme hypersensitivity.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
hypersensitive to UV; Not hypersensitive to UV-psoralen.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Ease_of_scoringES1_Very_hard_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000228Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkNon-Unc revertants of unc-58 were not observed from among 25 plates screened.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayGenotypeunc-58(e665)Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000276Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknot hypersensitive to X irradiationPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentX-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000392Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000498Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknot hypersensitive to MMSPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000520Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals were indistinguishable in appearance from wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000742Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentRecombination frequencies were measured using unlinked intervals, dpy-10 unc-4 or dpy-7 unc-3.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000784Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMale fertility was 85% that of wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001175Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001631Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMales produced equal numbers of X-bearing and nullo-X-bearing sperm.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
ReferenceWBPaper00000565
MethodAllele