WormBase Tree Display for Variation: WBVar00089075

WBVar00089075 Name: Public_name: mn159
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00034250
    Laboratory: SP
    Status: Live
  Affects: Gene: WBGene00000537
    Interactor (105)
  Genetics: Mapping_data: In_multi_point: 368
        369
  Description: Phenotype (12)
    Phenotype_not_observed: WBPhenotype:0000276 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00000565
          Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: X-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls. Paper_evidence: WBPaper00000565
              Curator_confirmed: WBPerson712
      WBPhenotype:0000386 Paper_evidence: WBPaper00035318
        Curator_confirmed: WBPerson2987
        Remark: Cobalt-induced germline cell apoptosis was unaffected in clk-2(mn159) mutants (Figure 2) Paper_evidence: WBPaper00035318
            Curator_confirmed: WBPerson2987
        Affected_by: Molecule: WBMol:00003058 Paper_evidence: WBPaper00035318
              Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: Synchronized young adult hermaphrodites were treated in K-medium with 0.01 millimolar Cobalt chloride for 12 hours, and apoptotic cells were scored after AO staining. Paper_evidence: WBPaper00035318
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000392 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00000565
          Curator_confirmed: WBPerson712
      WBPhenotype:0000436 Paper_evidence: WBPaper00005597
        Curator_confirmed: WBPerson712
        Remark: HUS-1::GFP localization was not different in rad-5(mn159) than in the parental strain. Paper_evidence: WBPaper00005597
            Curator_confirmed: WBPerson712
      WBPhenotype:0000498 Paper_evidence: WBPaper00000565
        Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: Not hypersensitive to MMS. Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00000565
          Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood. Paper_evidence: WBPaper00000565
              Curator_confirmed: WBPerson712
      WBPhenotype:0000520 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
        Remark: Animals were indistinguishable in appearance from wild type. Paper_evidence: WBPaper00000565
            Curator_confirmed: WBPerson712
      WBPhenotype:0000742 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Recombination frequencies were measured using unlinked intervals, dpy-10 unc-4 or dpy-7 unc-3. Paper_evidence: WBPaper00000565
              Curator_confirmed: WBPerson712
      WBPhenotype:0001175 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
      WBPhenotype:0001631 Paper_evidence: WBPaper00000565
        Curator_confirmed: WBPerson712
        Remark: Males produced equal numbers of X-bearing and nullo-X-bearing sperm. Paper_evidence: WBPaper00000565
            Curator_confirmed: WBPerson712
        EQ_annotations: Life_stage: WBls:0000056 PATO:0000460 Paper_evidence: WBPaper00000565
                Curator_confirmed: WBPerson712
  Reference (19)
  Method: Allele