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WormBase Tree Display for Variation: WBVar00088037

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WBVar00088037	Name	Public_name	it112
		Other_name	it112ts
	Sequence_details	SeqStatus	Pending_curation
	Variation_type	Allele
	Origin	Species	Caenorhabditis elegans
		Strain	WBStrain00000398
			WBStrain00000399
		Laboratory	KK
		Status	Live
	Affects	Gene	WBGene00004972
	Genetics	Mapping_data	In_2_point	7046
	Description	Phenotype	WBPhenotype:0000670	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	Mutants produce about half the normal number of spermatids compared to controls. Even fewer were produced when tested over deficiency, mDf7. The spermatids that are made appear normal in shape, although they vary in size more than wild type.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
			WBPhenotype:0000692	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	spe-26 males are sterile.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
			WBPhenotype:0000981	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	Spermatocytes fail to complete meiosis; they remain as large, round cells with a granular appearance under differential interference contrast microscopy, and they fail to form spermatids. All defective spermatocytes showed multiple DAPI-positive regions. Nearly half had four condensed DAPI-positive regions, the remaining spermatocytes had more than four DAPI staining regions. Staining intensity was variable. Many spermatocytes showed normal-looking spindles.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				EQ_annotations	Anatomy_term	WBbt:0006799	PATO:0000460	Paper_evidence	WBPaper00002181
								Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
			WBPhenotype:0001300	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	In spe-26 mutant spermatocytes, the fibrous body-membranous organelle (FB-MO) complex disassembles in the spermatocytes themselves and the major sperm protein (MSP) filaments disperse. The MO membranes do fold into a compact structure, but they become distended. Spermatocytes show multiple nuclei by electron microscopy and mislocalization of the endoplasmic reticulum (ER) and of ribosomes.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				EQ_annotations	Anatomy_term	WBbt:0006799	PATO:0000460	Paper_evidence	WBPaper00002181
								Curator_confirmed	WBPerson712
					GO_term	GO:0005783	PATO:0000628	Paper_evidence	WBPaper00002181
								Curator_confirmed	WBPerson712
						GO:0005840	PATO:0000628	Paper_evidence	WBPaper00002181
								Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
			WBPhenotype:0001346	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	In spe-26 mutant spermatocytes stained with rhodamine-conjugated phalloidin, actin filaments were seen aggregated to one side of the undivided cells instead of accumulated in the center.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				EQ_annotations	Anatomy_term	WBbt:0006799	PATO:0000460	Paper_evidence	WBPaper00002181
								Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
			WBPhenotype:0001360	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	Mutant hermaphrodites lay unfertilized oocytes. Oocytes can be fertilized by wild-type male sperm. Mutants are completely sterile at 25C but produce various numbers of progeny at 20C and 16C.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Recessive	Paper_evidence	WBPaper00002181
					Curator_confirmed	WBPerson712
				Variation_effect	Hypomorph_reduction_of_function	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
				Temperature_sensitive	Heat_sensitive		Paper_evidence	WBPaper00002181
							Curator_confirmed	WBPerson712
		Phenotype_not_observed	WBPhenotype:0000390	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	Spermatids can be activated in vitro to produce spermatozoa with motile pseudopods (data not shown).	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
			WBPhenotype:0000648	Paper_evidence	WBPaper00002181
				Curator_confirmed	WBPerson712
				Remark	spe-26 males appear to mate normally.	Paper_evidence	WBPaper00002181
						Curator_confirmed	WBPerson712
	Reference	WBPaper00015757
		WBPaper00002181
	Method	Allele