WormBase Tree Display for Variation: WBVar00087802

WBVar00087802 Evidence: Paper_evidence: WBPaper00002181
  Name: Public_name: hc139
    Other_name: hc139ts
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00000396
      WBStrain00000402
    Laboratory: BA
    Status: Live
  Affects: Gene: WBGene00004972
    Interactor: WBInteraction000519006
  Genetics: Mapping_data: In_2_point: 7044
  Description: Phenotype: WBPhenotype:0000670 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: Mutants produce almost no spermatids. Phenotype was unchanged when tested over deficiency, mDf7. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
      WBPhenotype:0000692 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: spe-26 males are sterile. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
      WBPhenotype:0000981 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: Spermatocytes fail to complete meiosis; they remain as large, round cells with a granular appearance under differential interference contrast microscopy, and they fail to form spermatids. All defective spermatocytes showed multiple DAPI-positive regions. Nearly half had four condensed DAPI-positive regions, the remaining spermatocytes had more than four DAPI staining regions. Staining intensity was variable. Many spermatocytes showed normal-looking spindles. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006799 PATO:0000460 Paper_evidence: WBPaper00002181
                Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
      WBPhenotype:0001300 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: In spe-26 mutant spermatocytes, the fibrous body-membranous organelle (FB-MO) complex disassembles in the spermatocytes themselves and the major sperm protein (MSP) filaments disperse. The MO membranes do fold into a compact structure, but they become distended. Spermatocytes show multiple nuclei by electron microscopy and mislocalization of the endoplasmic reticulum (ER) and of ribosomes. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006799 PATO:0000460 Paper_evidence: WBPaper00002181
                Curator_confirmed: WBPerson712
          GO_term: GO:0005783 PATO:0000628 Paper_evidence: WBPaper00002181
                Curator_confirmed: WBPerson712
            GO:0005840 PATO:0000628 Paper_evidence: WBPaper00002181
                Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
      WBPhenotype:0001346 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: In spe-26 mutant spermatocytes stained with rhodamine-conjugated phalloidin, actin filaments were seen aggregated to one side of the undivided cells instead of accumulated in the center. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006799 PATO:0000460 Paper_evidence: WBPaper00002181
                Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
      WBPhenotype:0001360 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: Mutant hermaphrodites lay unfertilized oocytes. Oocytes can be fertilized by wild-type male sperm. Mutants are completely sterile at 25C but produce various numbers of progeny at 20C and 16C. Temperature-sensitive period is around the L4 to adult molt in both shift-up and shift-down experiments. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00002181
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00002181
              Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000648 Paper_evidence: WBPaper00002181
        Curator_confirmed: WBPerson712
        Remark: spe-26 males appear to mate normally. Paper_evidence: WBPaper00002181
            Curator_confirmed: WBPerson712
  Reference: WBPaper00002181
  Method: Allele