WormBase Tree Display for Variation: WBVar00087750
expand all nodes | collapse all nodes | view schema
WBVar00087750 | Name | Public_name | hc17 | ||||||
---|---|---|---|---|---|---|---|---|---|
Other_name | hc17ts | ||||||||
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain (11) | |||||||||
Component_of_genotype | WBGenotype00000033 | ||||||||
Laboratory | BA | ||||||||
CF | |||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00001411 | |||||||
Interactor (11) | |||||||||
Genetics | Mapping_data | In_multi_point | 114 | ||||||
Description | Phenotype | WBPhenotype:0000113 | Paper_evidence | WBPaper00031903 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Expression of 21 U RNAs was reduced although expression of miRNA let-7 was unaffected. | Paper_evidence | WBPaper00031903 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Probable_null_via_phenotype | Paper_evidence | WBPaper00031903 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000038 | PATO:0000460 | Paper_evidence | WBPaper00031903 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Samples were enriched for RNAs <200 bp and used in northern analysis to examine the expression profile of the 21U-RNA 21ur-1 as | Paper_evidence | WBPaper00031903 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000114 | Paper_evidence | WBPaper00039871 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | vit-2 and vit-5 mRNA were significantly reduced on adult days 1 and 2 compared to wildtype, whereas the levels of sod-3 mRNA were roughly normal. Levels of vit-2 mRNA were elevated in mated hermaphrodites in contrast to unmated hermaphrodites, whereas mating had no effect on sod-3 mRNA. | Paper_evidence | WBPaper00039871 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000136 | Paper_evidence | WBPaper00031962 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Levels of prg-1 mRNA were almost 2-fold greater than in wild-type animals as assayed by qRT-PCR. | Paper_evidence | WBPaper00031962 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000137 | Paper_evidence | WBPaper00034714 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | elt-1 was not detectably expressed in the germ line of fem-1 adults | Paper_evidence | WBPaper00034714 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Variation_effect | Probable_null_via_phenotype | Paper_evidence | WBPaper00034714 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Temperature_sensitive | Heat_sensitive | Paper_evidence | WBPaper00034714 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Phenotype_assay | Treatment | In situ hybridization with elt-1 antisense probe | Paper_evidence | WBPaper00034714 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0000290 | Paper_evidence | WBPaper00031961 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000670 | Paper_evidence | WBPaper00031962 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals were devoid of sperm. | Paper_evidence | WBPaper00031962 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000687 | Paper_evidence | WBPaper00000724 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPerson712 | |||||||||
Remark | hc17 is a weaker fem-1 mutation. The feminization phenotype is restricted to the gonads of XX and XO animals. | Paper_evidence | WBPaper00000724 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
causes only partial XO feminization at 25C, XX self fertile at 20C | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Penetrance | Incomplete | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000724 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | Paper_evidence | WBPaper00000724 | ||||
Curator_confirmed | WBPerson2021 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00000724 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000688 | Paper_evidence | WBPaper00031903 | |||||||
WBPaper00032067 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Penetrance | Complete | Paper_evidence | WBPaper00031903 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Probable_null_via_phenotype | Paper_evidence | WBPaper00031903 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00031903 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00031903 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001410 | Paper_evidence | WBPaper00040337 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Antibodies that recognize the nearly identical GSP-3 and GSP-4 (GSP-3/4) proteins were used to show that, similar to the sperm-specific MSP protein, GSP-3/4 are expressed only in sperm-producing fem-3(q20gf) but not oocyte-producing fem-1(hc17lf) mutant hermaphrodites. | Paper_evidence | WBPaper00040337 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001759 | Paper_evidence | WBPaper00031961 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | 21U-RNA-1 and 21U-RNA-3442 RNAs were greatly reduced from RNA preparations from populations at 25C. Whereas, 21U-RNAs were expressed at approximately equal levels in male or female-enriched populations of wild-type animals. | Paper_evidence | WBPaper00031961 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00032067 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibited life span characteristics similar to wild-type. | Paper_evidence | WBPaper00032067 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000394 | Paper_evidence | WBPaper00031961 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | PLG-1 was detectable in Western blots of protein lysates. | Paper_evidence | WBPaper00031961 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000416 | Paper_evidence | WBPaper00039871 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Yolk protein levels were normal to slightly elevated in unmated animals. | Paper_evidence | WBPaper00039871 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001740 | Paper_evidence | WBPaper00032067 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Based on an aging model built on pharynx images from adults days 0-12. In this study fem-1(hc17) was the control. | Paper_evidence | WBPaper00032067 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003681 | PATO:0000460 | Paper_evidence | WBPaper00032067 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000041 | PATO:0000460 | Paper_evidence | WBPaper00032067 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00032067 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001759 | Paper_evidence | WBPaper00031962 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | 21UR-1 levels were unchanged at the restrictive temperature compared to control animals. | Paper_evidence | WBPaper00031962 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | GO_term | GO:0034585 | PATO:0000460 | Paper_evidence | WBPaper00031962 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00031962 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001779 | Paper_evidence | WBPaper00035664 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | Expression of miRNAs 35-41 is unaffected | Paper_evidence | WBPaper00035664 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Temperature_sensitive | Heat_sensitive | Paper_evidence | WBPaper00035664 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Reference (25) | |||||||||
Method | Allele |