Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00000460

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00000460EvidencePaper_evidenceWBPaper00048626
NamePublic_namebn2
Other_nameCE24685:p.Gly296Asp
Y87G2A.5.1:c.887G>A
HGVSgCHROMOSOME_I:g.13556729C>T
Sequence_detailsSMapS_parentSequenceY87G2A
Flanking_sequencestgcccggatacgataaaaaaatcgaattcgcgtgctcaactcgttcgcctacaagattca
Mapping_targetY87G2A
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00048626
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00000261
WBStrain00030635
WBStrain00030636
WBStrain00034434
WBStrain00056203
WBStrain00056208
WBStrain00056254
Component_of_genotypeWBGenotype00000069
WBGenotype00000159
LaboratorySS
SJZ
StatusLive
Linked_toWBVar02144875
WBVar02144876
WBVar02144874
AffectsGeneWBGene00006936
TranscriptY87G2A.5.1 (12)
Interactor (18)
GeneticsGene_classglp
Interpolated_map_positionI21.4559
Mapping_dataIn_2_point6106
6107
In_multi_point2176
2177
3241
3242
3380
3429
In_pos_neg_data6688
6689
6690
6691
6692
6693
6694
DescriptionPhenotypeWBPhenotype:0000038Paper_evidenceWBPaper00050044
Curator_confirmedWBPerson17144
RemarkIncrease rupture post-reproductivelyPaper_evidenceWBPaper00050044
Curator_confirmedWBPerson17144
WBPhenotype:0000120Paper_evidenceWBPaper00027619
Curator_confirmedWBPerson2021
RemarkCPB-3 was barely detected in an extract of glp-4(bn2) adult animals cultured at the restrictive temperaturePaper_evidenceWBPaper00027619
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00027619
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentanti-CPB-3 stainingPaper_evidenceWBPaper00027619
Curator_confirmedWBPerson2021
WBPhenotype:0000134Paper_evidenceWBPaper00040043
Curator_confirmedWBPerson712
Remarkcki-2 mRNA is absent from germline-less glp-4(bn2ts) animals.Paper_evidenceWBPaper00040043
Curator_confirmedWBPerson712
WBPhenotype:0000137Paper_evidenceWBPaper00031962
WBPaper00004137
Curator_confirmedWBPerson712
WBPerson2021
RemarkExpression of prg-2 mRNA was severely reduced compared with levels in wild-type as demonstrated by quantitative RT-PCR on total RNA isolated from 12-hour adult animals grown at 25C.Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
ego-1 transcripts were barely detectable in glp-4 mutantsPaper_evidenceWBPaper00004137
Curator_confirmedWBPerson2021
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00004137
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
25CPaper_evidenceWBPaper00004137
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentNorthern blot analysisPaper_evidenceWBPaper00004137
Curator_confirmedWBPerson2021
Temperature25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
WBPhenotype:0000202Paper_evidenceWBPaper00035486
Curator_confirmedWBPerson3490
Phenotype_assayGenotypeapl-1::gfp::unc-54(3'UTR)Paper_evidenceWBPaper00035486
Curator_confirmedWBPerson3490
WBPhenotype:0000215Paper_evidenceWBPaper00031961
WBPaper00005597
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00005597
Curator_confirmedWBPerson712
WBPhenotype:0000313Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkGerm cells arrested at meiotic prophase.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000394Paper_evidenceWBPaper00031961
Curator_confirmedWBPerson712
RemarkPLG-1 was not detectable in Western blots of protein lysates.Paper_evidenceWBPaper00031961
Curator_confirmedWBPerson712
WBPhenotype:0000396Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknon-reflexed gonad armsPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000688Paper_evidenceWBPaper00064734
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkhermaphrodites raised at 25C sterile,~12 germ line nuclei, males at 25C ~16 germ line nuclei, normal soma. Fertile at 16C; TSP throughout larval developmentPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000812Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
RemarkAnimals are devoid of germ cells.Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
WBPhenotype:0000823Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkhermaphrodites raised at 25C sterile,~12 germ line nuclei, males at 25C ~16 germ line nuclei, normal soma. Fertile at 16C; TSP throughout larval developmentPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001014Paper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
Remarkglp-4 mutants enhanced nematode survival in the presence of the pathogen Pseudomonas aeruginosa and S. marcescensPaper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
WBPhenotype:0001183Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-4(bn2ts) mutants exhibited reduced fat content at the non-permissive temperature of 25 degrees Celsius, compared to wild type controls (Figure 1D-G)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTreatment250 microliters of Nile Red (Molecular Probes) solution (1 microgram per milliliter in 1 X PBS) or C1-BODIPYC12 (Molecular Probes) solution (2 micromolar in 1 X PBS) was applied to the surface of NGM (Nematode Growth Media) plates (~12 milliliters of agar) seeded with E. coli OP50. Plates were used immediately after dry in a laminar flow hood (for C1-BODIPY-C12 staining) or allowed to equilibrate overnight at room temperature (for Nile Red staining). For Nile Red staining, synchronized animals were grown on Nile Red-containing plates since L1 larval stage and taken pictures at adulthood. For C1-BODIPY-C12 staining, animals were transferred from NGM plates onto C1-BODIPY-C12-containing plates at L4 larval stage and taken pictures at adulthood.Quantification of Nile Red and C1-BODIPY-C12 staining was performed according to Mak et al (1). Fluorescence images were captured using a Zeiss Axioplan microscope and a CCD camera (Hamamatsu, ORCA-ER). To quantify fluorescence intensity, images of the anterior intestine were captured from animals whose anterior intestine was not covered by the gonad. The images were collected at 12-bit with the raw pixel values within the linear range of the CCD camera that is controlled by an "automatic exposure" function with the Openlab software (Improvision). To calculate the fluorescence intensity, the mean pixel intensity of all fluorescence staining lipid droplets in the first 3 pairs of intestinal cells were divided by the exposure time (Openlab, Improvision). For each genotype, the average of 15~20 animals was presented.Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Temperature25Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0001759Paper_evidenceWBPaper00031962
WBPaper00031961
Curator_confirmedWBPerson712
Remark21UR-1 was absent from animals at the restrictive temperature, which are devoid of germ cells.Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
21U-RNA-1 and 21U-RNA-3442 RNAs were absent from RNA preparations, whereas, 21U-RNAs were expressed at approximately equal levels in male or female-enriched populations of wild-type animals.Paper_evidenceWBPaper00031961
Curator_confirmedWBPerson712
EQ_annotationsGO_termGO:0034585PATO:0000460Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00031962
Curator_confirmedWBPerson712
WBPhenotype:0001776Paper_evidenceWBPaper00031961
WBPaper00035324
Curator_confirmedWBPerson712
WBPerson2021
RemarkAnimals exhibited a severe depletion of endogenous siRNAs.Paper_evidenceWBPaper00031961
Curator_confirmedWBPerson712
The glp-4 mutant exhibits an approximately 50% decline in 21-nt siRNA expression, but a complete loss of 26G RNAsPaper_evidenceWBPaper00035324
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00035324
Curator_confirmedWBPerson2021
WBPhenotype:0001779Paper_evidenceWBPaper00035664
Curator_confirmedWBPerson2021
RemarkGonadless animals fail to express miRNAs 35-41 at levels detectable by northern blotsPaper_evidenceWBPaper00035664
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00035664
Curator_confirmedWBPerson2021
WBPhenotype:0001823Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
RemarkMale sperm failed to accumulate in the spermatheca in glp-4(bn2) mutants at the restrictive temperaturePaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentWild-type males labelled with MitoTracker were mated to non-labelled mutant hermaphroditesPaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
WBPhenotype:0001861Paper_evidenceWBPaper00005597
Curator_confirmedWBPerson712
RemarkWith a missing germline, induction of germline expressed transcripts is abrogated, as in the case with irradiation induced egl-1. In contrast to wild-type worms, bn2 mutants show a significant decrease in induction of egl-1 in response to X-ray irradiation.Paper_evidenceWBPaper00005597
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00005597
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001182Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Remarkglp-4(bn2ts) mutants exhibited normal fat content at the permissive temperature of 15 degrees Celsius, compared to wild type controls, as determined by Nile Red staining (Figure S3C,D)Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
Phenotype_assayTemperature15Paper_evidenceWBPaper00032310
Curator_confirmedWBPerson2987
WBPhenotype:0001266Paper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
RemarkNematodes containing a mutation in either glp-1 or glp-4 did not have substantially more phospho-PMK-1 than did wild-type nematodesPaper_evidenceWBPaper00035490
Curator_confirmedWBPerson2021
WBPhenotype:0001426Paper_evidenceWBPaper00004883
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00004883
Curator_confirmedWBPerson712
Phenotype_assayGenotypearIs37Paper_evidenceWBPaper00004883
Curator_confirmedWBPerson712
WBPhenotype:0002534Paper_evidenceWBPaper00005130
Curator_confirmedWBPerson712
RemarkThe accumulation of both abf-1 and abf-2 transcripts was almost identical in adults of N2 and glp-4(bn2), and the eggs.Paper_evidenceWBPaper00005130
Curator_confirmedWBPerson712
Reference (36)
MethodSubstitution_allele