WormBase Tree Display for Transgene: WBTransgene00025454
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| WBTransgene00025454 | Public_name | WBPaper00003644_Ex2 | |||||
|---|---|---|---|---|---|---|---|
| Summary | Cel-unc-54(prom)::Cbr-her-1(cDNA)::Cel-her-1(3'UTR) + rol-6(su1006) | ||||||
| Construction | Construct | WBCnstr00040210 | |||||
| Coinjection | WBCnstr00004720 | ||||||
| Laboratory | BW | ||||||
| Construction_summary | "To observe effects of overexpression from cloned her-1 genes, DNA of the experimental plasmid was coinjected with the pRF4 plasmid carrying the rol-6(su1006dm) marker, each at a concentration of 100 μg/ml (Mello et al. 1991), to yield transgenic lines carrying extrachromosomal arrays." | ||||||
| Genetic_information | Extrachromosomal | ||||||
| Phenotype | WBPhenotype:0001572 | Paper_evidence | WBPaper00003644 | ||||
| Curator_confirmed | WBPerson629 | ||||||
| Remark | Ectopic expression: XX animals are masculinised. Both, Cel-her-1 and Cbr-her-1 cDNA expressed from transgenes masculinise both, C. elegans and C. briggsae. | Paper_evidence | WBPaper00003644 | ||||
| Curator_confirmed | WBPerson629 | ||||||
| Caused_by_gene | WBGene00038586 | Paper_evidence | WBPaper00003644 | ||||
| Curator_confirmed | WBPerson629 | ||||||
| Reference | WBPaper00003644 | ||||||
| Species | Caenorhabditis elegans |
