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WormBase Tree Display for Transgene: WBTransgene00025260

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Name Class

WBTransgene00025260Public_namenpEx14
Summary[pNP53 (mec-3p::ceh-17) + pRF4]
ConstructionConstructWBCnstr00039986
CoinjectionWBCnstr00004720
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0002490Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
RemarkWhen we ectopically expressed ceh-17 in the MCs, the processes of the AVM, PLM and ALM neurons started their normal trajectory, but they all failed to arrest their outgrowth at the usual position, and continued instead, often for a substantial distance (Fig. 4C,D,G,H and Table 1). For example, the PLM axon reached a position anterior to the AVM cell body in more than 20% of cases, and was occasionally more than twice its normal length, reaching as far as the nerve ring (Fig. 4G,I and Table 1). In the case of the AVM axon, most often its exaggerated anteriorward progress was stopped only when it reached the nose, whereupon it frequently turned back and grew posteriorly (Fig. 4C,D). Another phenotype is the growth of a posteriorly directed axon from the ALM cell body, which could be seen in 24% of the cases as opposed to 2% in the wild type (Fig. 4G,H and Table 1). These worms did not display any blatant mechanoreception deficit (data not shown), arguing that ectopic ceh-17 expression left untouched many aspects of the mechanoreceptor phenotype. Deletion of the homeodomain abolished the effects of the transgene (Fig. 4I and Table 1), strongly arguing that ectopic CEH-17 acts in mechanoreceptors through its transcriptional properties on specific promoter sequences.Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
Caused_by_geneWBGene00000440Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
EQ_annotationsAnatomy_termWBbt:0003832PATO:0000460Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
WBbt:0005490PATO:0000460Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
WBbt:0005406PATO:0000460Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
GO_termGO:0030424PATO:0000460Paper_evidenceWBPaper00004283
Curator_confirmedWBPerson499
ReferenceWBPaper00004283
SpeciesCaenorhabditis elegans