WormBase Tree Display for Transgene: WBTransgene00023300
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WBTransgene00023300 | Summary | [DAF-16a::GFP] | ||||||
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Construction | Construct | WBCnstr00023157 | ||||||
Laboratory | HT | |||||||
Construction_summary | integrated, high-copy | |||||||
Genetic_information | Phenotype | WBPhenotype:0000012 | Paper_evidence | WBPaper00036474 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Transgenic DAF-16a fully rescues the dauer defective phenotype of daf-16(mgDf50) (Figure 2C, Table S4) | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00036474 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000061 | Paper_evidence | WBPaper00036474 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | High copy, integrated DAF-16a::GFP (Figure 1A, Table S1) | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
High copy, integrated DAF-16a::GFP (Figure S3A, Table S2) | Paper_evidence | WBPaper00036474 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
High copy, integrated DAF-16a::GFP (Figure S3B, Table S2) | Paper_evidence | WBPaper00036474 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | daf-16(mgDf50) | Paper_evidence | WBPaper00036474 | ||||
Curator_confirmed | WBPerson2987 | |||||||
daf-2(e1370);daf-16(mgDf50) | Paper_evidence | WBPaper00036474 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000295 | Paper_evidence | WBPaper00036474 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Transgenic DAF-16a partially rescues the reduced thermotolerance phenotype of daf-16(mgDf50) (Figure 2D, Table S4) | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Temperature | 37 | Paper_evidence | WBPaper00036474 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_not_observed | WBPhenotype:0001182 | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "We next examined the role of DAF-16 isoforms in fat storage by using Sudan Black25 and Oil-Red-O staining. daf-16d/f transgenic worms stored more fat in the intestine whereas daf-16a transgenic worms were comparable to daf-16(mgDf50); daf-2(e1370) mutants (Fig 2e and Supplementary Fig 6). Thus DAF-16d/f has a major function in fat metabolism." | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00000912 | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | Animals were stained with Sudan Black to assess fat content | Paper_evidence | WBPaper00036474 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Genotype | daf-16(mgDf50); daf-2(e1370) | Paper_evidence | WBPaper00036474 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Reference | WBPaper00036474 | |||||||
Species | Caenorhabditis elegans |