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WormBase Tree Display for Transgene: WBTransgene00023300

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Name Class

WBTransgene00023300Summary[DAF-16a::GFP]
ConstructionConstructWBCnstr00023157
LaboratoryHT
Construction_summaryintegrated, high-copy
Genetic_informationPhenotypeWBPhenotype:0000012Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
RemarkTransgenic DAF-16a fully rescues the dauer defective phenotype of daf-16(mgDf50) (Figure 2C, Table S4)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00000912Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Phenotype_assayTemperature25Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
WBPhenotype:0000061Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
RemarkHigh copy, integrated DAF-16a::GFP (Figure 1A, Table S1)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
High copy, integrated DAF-16a::GFP (Figure S3A, Table S2)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
High copy, integrated DAF-16a::GFP (Figure S3B, Table S2)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00000912Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-16(mgDf50)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
daf-2(e1370);daf-16(mgDf50)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
WBPhenotype:0000295Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
RemarkTransgenic DAF-16a partially rescues the reduced thermotolerance phenotype of daf-16(mgDf50) (Figure 2D, Table S4)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00000912Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Phenotype_assayTemperature37Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0001182Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Remark"We next examined the role of DAF-16 isoforms in fat storage by using Sudan Black25 and Oil-Red-O staining. daf-16d/f transgenic worms stored more fat in the intestine whereas daf-16a transgenic worms were comparable to daf-16(mgDf50); daf-2(e1370) mutants (Fig 2e and Supplementary Fig 6). Thus DAF-16d/f has a major function in fat metabolism."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00000912Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals were stained with Sudan Black to assess fat contentPaper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Genotypedaf-16(mgDf50); daf-2(e1370)Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
ReferenceWBPaper00036474
SpeciesCaenorhabditis elegans