WormBase Tree Display for Transgene: WBTransgene00023140
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WBTransgene00023140 | Public_name | zfEx40 | ||||||
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Summary | Plgc-55::LGC-55 cation-II | |||||||
Construction | Construct | WBCnstr00022954 | ||||||
Laboratory | QW | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000315 | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)." | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00013746 | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | lgc-55(tm2913) | Paper_evidence | WBPaper00048522 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001005 | Paper_evidence | WBPaper00048522 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)." | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00013746 | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | lgc-55(tm2913) | Paper_evidence | WBPaper00048522 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002366 | Paper_evidence | WBPaper00048522 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)." | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00013746 | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | lgc-55(tm2913) | Paper_evidence | WBPaper00048522 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002399 | Paper_evidence | WBPaper00048522 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Exogenous tyramine induced neck muscle relaxation and lengthening of the head in wild-type (LGC-55 anion) animals and lgc-55 null mutant animals that express a rescuing LGC-55 anion transgene (wild type: Δhead = 10 6 μm, n = 68; Plgc-55:LGC-55(zfEx2): Δhead = 11 4 μm, n = 75) (Fig 5A and 5B). Head movements persisted in lgc-55 mutants [19], with no significant change in head length (lgc-55(tm2913): Δhead = 3 13 μm, n = 65). In contrast, transgenic animals that expressed the engineered LGC-55 cation-I or LGC-55 cation-II channel under control of the native promoter had a hypercontracted and shortened head length in response to exogenous tyramine (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -15 2 μm, n = 49; Plgc-55::LGC-55 cation- II (zfEx40): Δhead = -28 2 μm, n = 49) (Fig 5A and 5B)." | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
"In wild-type animals, exogenous tyramine also induced long backward runs preceding immobilization through the LGC-55 mediated inhibition of the AVB premotor interneurons that drive forward locomotion (wild type: Δfwd-bwd = -8.46 2.98 body bends, n = 40) (Fig 5A and 5D, S1 Movie) [19]. Backward locomotion was further increased in transgenic animals that expressed the LGC-55 anion under control of its endogenous promoter (Plgc-55:LGC-55 (zfEx2): Δfwd-bwd = -28.5 3.3 backward body bends, n = 29). lgc-55 null mutants did not make long reversals when exposed to exogenous tyramine (lgc-55(tm2913): Δfwd-bwd = 2.58 0.9 body bends, n = 34). In sharp contrast, LGC-55 cation animals exhibit long forward runs (Plgc-55::LGC-55 cation-I (zfEx8): Δfwd-bwd = 46.5 8.4 body bends, n = 28; Plgc-55::LGC-55 cation-II (zfEx40): Δfwd-bwd = 80.6 9.5 body bends, n = 39), which continued for an extended period of time (Fig 5A, 5C, and 5D; S2 Movie). The forward runs and head contractions were more pronounced in LGC-55 cation-II than in LGC-55 cation-I transgenic animals, supporting the notion that the R to D substitution at the extracellular ring of the M2 domain increases the cation conductance (Fig 5C and 5D)." | Paper_evidence | WBPaper00048522 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00013746 | Paper_evidence | WBPaper00048522 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00001396 | Paper_evidence | WBPaper00048522 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | lgc-55(tm2913) | Paper_evidence | WBPaper00048522 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Reference | WBPaper00048522 | |||||||
Species | Caenorhabditis elegans |