WormBase Tree Display for Transgene: WBTransgene00019937
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WBTransgene00019937 | Public_name | WBPaper00001762Ex1 | |
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Summary | [hsp16-lacZ; pRF4] | ||
Synonym | 48.1C | ||
PC6ubEx6 | |||
Construction | Construct | WBCnstr00019264 | |
Coinjection | WBCnstr00004720 | ||
Laboratory | PC | ||
Construction_summary | Clone = pPCZ1. Construct 48.1C contains the complete hsp16-48/1 gene pair and is an in-frame translational fusion of the lacZ coding region with the second exon of hsp16-1. 48.1C was constructed by inserting a 3500bp HindIII-AflI fragment encompassing the lacZ gene (nucleotides 18-3518 of the expression vector pPD16.43 received from A.Fire, Carnegie Institution) into the HpaI site (nucleotide 3565 of the published sequence) of the hspl6-1 gene. pPCZ1 contains a complete hspl6-48/1 gene pair extending from a BclI site at nucleotide 2280 to the BamHI site at nucleotide 4186 in the published sequence (Russnak and Candido,1985). | ||
Genetic_information | Extrachromosomal | ||
Associated_with | Strain | WBStrain00030547 | |
Reference | WBPaper00001497 | ||
WBPaper00001762 | |||
Species | Caenorhabditis elegans |