WormBase Tree Display for Transgene: WBTransgene00019702
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| WBTransgene00019702 | Public_name | qSi29 | |
|---|---|---|---|
| Summary | [Psygl-1(4XLBS mut)::H2B::GFP::sygl-1 3'end; unc-119(+)] | ||
| Construction | Construct | WBCnstr00019033 | |
| Coinjection_other | unc-119(+) | ||
| Integration_method | Single_copy_insertion | ||
| Construction_summary | For H2B::GFP reporter transgenes, a construct (pJK1634) containing ~2.2 kb of sequence upstream of the sygl-1 start driving expression of H2B::GFP coding sequence from pCM1.35 (Addgene; plasmid 17248), followed by the sygl-1 3'UTR and intergenic region was cloned into the SpeI site of pCFJ151 (Addgene; plasmid 19330). A separate clone (pJK1635) was generated identical to pJK1634 except that each of the four consensus LAG-1 binding sites was mutated from the wild-type consensus RTGGGAA to the mutant form RACGGAA using QuikChange Site Directed Mutagenesis Kit (Stratagene).The construct was integrated into the ttTi5605 site in LGII of strain EG4322. | ||
| Genetic_information | Integrated | ||
| Map | II | ||
| Associated_with | Strain | WBStrain00022659 | |
| Reference | WBPaper00044909 | ||
| Species | Caenorhabditis elegans |
