WormBase Tree Display for Transgene: WBTransgene00019325
expand all nodes | collapse all nodes | view schema
| WBTransgene00019325 | Public_name | oxEx1067 | |
|---|---|---|---|
| Summary | [SNF-3::GFP; Pmyo-3::mCherry; Punc-122::GFP] | ||
| Construction | Construct | WBCnstr00018662 | |
| Coinjection_other | Pmyo-3::mCherry | ||
| Punc-122::GFP | |||
| Construction_summary | To generate a fluorescently tagged SNF-3, we cut the GFP with MluI from the pPD114.24 vector (A. Fire, Stanford University), and ligated it into an MluI site in the largest intron in frame with the gene to generate pADA65. The GFP tag is located in the extracellular loop between transmembrane domain 9 and 10. 30 ng ul-1 of pADA65 was injected in snf-3(ox354) egl-8(sa47) along with 2 ng ul-1 Pmyo-3::mCherry and Punc-122::GFP co-injection marker. Multiple transgenic lines were obtained and they fully rescued snf-3(ox354) enhancer defects (strain EG4769 carries oxEx1067). | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr11367 | |
| Associated_with | Strain | WBStrain00042800 | |
| Reference | WBPaper00044443 | ||
| Species | Caenorhabditis elegans |
