WormBase Tree Display for Transgene: WBTransgene00019309
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| WBTransgene00019309 | Public_name | qaEx1410 | |
|---|---|---|---|
| Summary | [unc-36(+) hsp16-2::ptx] | ||
| Construction | Construct | WBCnstr00018647 | |
| Coinjection_other | unc-36(+) | ||
| Construction_summary | Clone = pCBD14 (lost). The ptx gene, encoding the catalytic subunit S1 of PTX, was amplified from plasmid pPTX42 (Locht and Keith, Science, 1986) by PCR using primers 5'-CGGGATCCATGGACGATCCTCCCGCCACCGTATACC and 5'- GGGGTACCCTAGAACGAATACGCGATGCTTTCGTAG. The PCR product lacked all nucleotides encoding the cleavable signal sequence except an ATG to encode an initial methionine. Restriction sites for BamHI and KpnI were included in the primers, and the product was cloned using those enzymes in the C. elegans expression vector pPD49.78. The insert in the resulting plasmid, pCBD14, was verified by DNA sequencing. XA1413, the strain carrying this transgene, has been lost. | ||
| Genetic_information | Extrachromosomal | ||
| Associated_with | Strain | WBStrain00046724 | |
| Reference | WBPaper00004862 | ||
| Species | Caenorhabditis elegans |
