WormBase Tree Display for Transgene: WBTransgene00018801
expand all nodes | collapse all nodes | view schema
| WBTransgene00018801 | Public_name | WBPaper00003631Ex1 | |
|---|---|---|---|
| Summary | [Pegl-1::gfp; lin-15(+)] | ||
| Synonym | Expr11092_Ex | ||
| Construction | Construct | WBCnstr00018162 | |
| Coinjection_other | pL15-EK | ||
| Construction_summary | Clone = pBC99. To construct Pegl-1::gfp, bases +174 to +5820 (5'-3') downstream of the stop codon of the egl-1 gene and bases -1914 to -837 (5'-3') upstream of the stop codon were amplified with appropriate primers and cloned into the SpeI-ApaI (5'-3') and PstI-BamHI (5'-3') sites of vector pPD95.69, respectively (A. Fire et al., personal communication). --precise ends. | ||
| a green fluorescent protein (GFP) reporter construct in which the expression of the gfp gene is under the control of the 5' (1.0 kb upstream of the 5' UTR of egl-1) and 3' (5.6 kb downstream of the 3' UTR of egl-1) regulatory regions of the egl-1 gene (Pegl-1::gfp). | |||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr11092 | |
| Reference | WBPaper00003631 | ||
| Species | Caenorhabditis elegans |
