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WormBase Tree Display for Transgene: WBTransgene00018796

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Name Class

WBTransgene00018796Public_nameWBPaper00042543Ex1
Summary[Pcyc-2.1::GFP; Punc-122::GFP]
SynonymExpr11086_Ex
ConstructionConstructWBCnstr00018157
CoinjectionWBCnstr00005331
Construction_summaryClone = pJG03. Approximately 1 kb upstream of the ATG start codon of cyc-2.1 were amplified by PCR (Forward primer, AATTGTCGACCCACTTCGCCTAAGTTGCGG; Reverse primer, AATTACCGGTGTTGAACCCTTAAAATACAG). This fragment containing the putative endogenous promoter of the cyc-2.1 gene was cloned upstream of the GFP encoding sequence in pPD95.75 (kindly provided by A. Fire, Stanford University School of Medicine), resulting in plasmid pJG03. --precise ends.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr11086
ReferenceWBPaper00042543
SpeciesCaenorhabditis elegans