WormBase Tree Display for Transgene: WBTransgene00018643
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WBTransgene00018643 | Public_name | bhEx72 | |
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Summary | [hda-1::gfp + unc-119(+)] | ||
Construction | Construct | WBCnstr00018008 | |
Coinjection_other | unc-119(+) | ||
Construction_summary | Clone = pGLC44. This hda-1::gfp plasmid was made by subcloning an NsiI/HindIII-digested 1,024 bp DNA fragment from C. elegans into the Fire lab vector pPD95.69 (polymerase chain reaction primers GL363/GL360). Because the C. elegans fragment contains all but approximately 250 bp of the DNA region between hda-1 and its upstream gene, ril-1, it is formally possible that gfp expression in the transgenic animals is regulated by both the hda-1 and ril-1 enhancers. However, this is unlikely because ril-1 has no known function in vulva and uterine cells (HANSEN et al. 2005; LEMIRE et al. 2009). | ||
Genetic_information | Extrachromosomal | ||
Used_for | Expr_pattern | Expr11136 | |
Reference | WBPaper00043890 | ||
Species | Caenorhabditis elegans |