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WormBase Tree Display for Transgene: WBTransgene00018643

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Name Class

WBTransgene00018643Public_namebhEx72
Summary[hda-1::gfp + unc-119(+)]
ConstructionConstructWBCnstr00018008
Coinjection_otherunc-119(+)
Construction_summaryClone = pGLC44. This hda-1::gfp plasmid was made by subcloning an NsiI/HindIII-digested 1,024 bp DNA fragment from C. elegans into the Fire lab vector pPD95.69 (polymerase chain reaction primers GL363/GL360). Because the C. elegans fragment contains all but approximately 250 bp of the DNA region between hda-1 and its upstream gene, ril-1, it is formally possible that gfp expression in the transgenic animals is regulated by both the hda-1 and ril-1 enhancers. However, this is unlikely because ril-1 has no known function in vulva and uterine cells (HANSEN et al. 2005; LEMIRE et al. 2009).
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr11136
ReferenceWBPaper00043890
SpeciesCaenorhabditis elegans