WormBase Tree Display for Transgene: WBTransgene00018359
expand all nodes | collapse all nodes | view schema
| WBTransgene00018359 | Public_name | WBPaper00005184Ex1 | |
|---|---|---|---|
| Summary | [Pptp-1::PTP-1::GFP] | ||
| Synonym | Expr10816_Ex | ||
| [ptp-ferm::ptp-ferm-gfp] | |||
| Construction | Construct | WBCnstr00017744 | |
| Construction_summary | Clone = 95.P1. The expression plasmid, 95.P1, containing the ptp-ferm::ptp-ferm-gfp chimeric mini gene was constructed as follows. A 7 kb 5' genomic fragment of ptp-ferm (nt -5880-1145) was generated by PCR using the C48D5 cosmid as a template and primers, 5'-aagcatgcagttcctctagccaatcgcataa-3' and 5'-aacccgggtagaattggaacctcgtaaactcgtc- 3'. The DNA fragment was digested by SphI and SmaI, and cloned into the SphI/SmaI site of pPD95.77, a gfp reporter vector developed by A. Fire at the Carnegie Institution of Washington. The resulting plasmid, pPD95/PTP-FERM/GFP1, was digested with BamHI/BalI and ligated to a PTP-FERM cDNA fragment. The resulting plasmid, 95.P1, was used to express PTP-FERM-GFP in the worm. -- precise ends. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr10816 | |
| Reference | WBPaper00005184 | ||
| Species | Caenorhabditis elegans |
