WormBase Tree Display for Transgene: WBTransgene00016142
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| WBTransgene00016142 | Public_name | WBPaper00003761Ex7 | |
|---|---|---|---|
| Summary | [pCHE-2::GFP3] | ||
| Construction | Construct | WBCnstr00015708 | |
| Construction_summary | pCHE-2::GFP3 was made by removing the KpnI cassette containing the nuclear localization signal from pCHE-2::GFP2. pCHE-2::GFP2 was made by ligating the 5.4-kb EcoT14I-EcoRV fragment of the cosmid F38G1 (which contained a 650-bp 5' noncoding region and most of the coding region of che-2 gene) and the rest of the coding sequence from che-2 cDNA, into the pPD95.69 GFP vector containing a nuclear localization signal (A. Fire, J. Ahnn, G. Seydoux and S. Xu, personal communication). The DNA sequence at the 3' end of the insert had been modified mainly by PCR, so that the C-terminal amino acid Asp of CHE-2 was changed to Lys followed by a linker of six amino acids. This construct had a 650-bp 5' upstream sequence and all the coding sequence. | ||
| Genetic_information | Extrachromosomal | ||
| Reference | WBPaper00003761 | ||
| Species | Caenorhabditis elegans |
