WormBase Tree Display for Transgene: WBTransgene00008988
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| WBTransgene00008988 | Public_name | cgIs60 | |||||
|---|---|---|---|---|---|---|---|
| Summary | [Pdat-1::(gpi-)PrP; Pdat-1::GFP] | ||||||
| Construction | Construct | WBCnstr00008679 | |||||
| Integration_method | UV | ||||||
| Construction_summary | The coding sequence for mouse PrP(1-254) carrying a human sequence-based 3F4 epitope was PCR amplified from the PrP1-254-mPrP1,28 using the following primers: 5'-GCG CGG CTA GCA TGG CGA ACC TTG GCT ACT GG-3' (forward), 5'-GCG CGG AGC TCT CAT CCC ACG ATC AGG AAG ATG A-3' (reverse). The resulting PCR products were digested with NheI/SacI and ligated to a plasmid that had been predigested with NheI/SacI: pRB490, containing the dopamine neuronal specific promoter Pdat-1,29 to create Pdat-1::PrP(1-254). | ||||||
| Genetic_information | Integrated | ||||||
| Phenotype_not_observed | WBPhenotype:0000882 | Paper_evidence | WBPaper00037809 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals exhibited normal neuron morphology. | Paper_evidence | WBPaper00037809 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | Anchorless form of mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0004028 | Paper_evidence | WBPaper00037809 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Animals exhibited normal sensing ability. | Paper_evidence | WBPaper00037809 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_other | Anchorless form of mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Reference | WBPaper00037809 | ||||||
| Species | Caenorhabditis elegans |
