WormBase Tree Display for Transgene: WBTransgene00008742
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| WBTransgene00008742 | Public_name | cwrIs722 | |
|---|---|---|---|
| Summary | [Pdat-1::GFP; Pdat-1::LRRK2(WT); lin-15(+)] | ||
| Synonym | LRRK2+ | ||
| Construction | Construct | WBCnstr00008442 | |
| Coinjection_other | lin-15(+) | ||
| Integration_method | UV | ||
| Construction_summary | Human cDNA encoding full-length C-terminally FLAG-tagged human LRRK2 WT was previously constructed in pCEP4 vector as described (Guo et al., 2007). The Pdat-1 promoter sequence was PCR amplified from the upstream 719-bp region containing the initiation codon ATG using the corresponding Promoterome clone (Geneservice, UK) as the template. The plasmid pFXneEGFP used previously as a C. elegans expression vector (Kuwahara et al., 2006) was modified by switching the original BamHI site to the region 17-bp downstream of the NotI site. The PCR product of Pdat-1 was subsequently inserted into KpnI and HindIII sites immediately upstream of the enhanced green fluorescent protein (GFP) of the modified pFXneEGFP to create Pdat-1::GFP (serving as a fluorescent reporter for DA neurons). The FLAG-tagged human LRRK2 cDNA was excised from NotI and BamHI sites of pCEP4 and subcloned into the same sites in the Pdat-1-containing pFXneEGFP, generating Pdat-1::LRRK2. | ||
| Genetic_information (2) | |||
| Reference | WBPaper00036154 | ||
| WBPaper00051457 | |||
| WBPaper00051539 | |||
| Species | Caenorhabditis elegans |
