WormBase Tree Display for Transgene: WBTransgene00008233
expand all nodes | collapse all nodes | view schema
| WBTransgene00008233 | Public_name | heIs30 | |
|---|---|---|---|
| Summary | [Prnr-1::cyb-1DesBox::3XVenus] | ||
| Construction | Construct | WBCnstr00007949 | |
| Coinjection_other | lin-48::TdTomato | ||
| Construction_summary | Prnr::CYB-1DesBox::3XVenus was created by cloning a tandem C. elegans optimized Venus (a kind gift of T. Ishihara, Kyushu University, Kyushu, Japan) in-frame with an N-terminal fragment of C. elegans cyb-1 cyclin B1 (nucleotides 1-321). This CYB-1DesBox fragment contains a putative destruction box for APC-dependent degradation. The codon usage was altered (optimized) to prevent co-suppression of the endogenous cyb-1 gene. CYB-1DesBox was expressed as a translational fusion with tripleVenus, controlled by the rnr-1 ribonucleotide reductase promoter in the pVT501 vector (a kind gift of V. Ambros). | ||
| Genetic_information | Integrated | ||
| Reference | WBPaper00037904 | ||
| Species | Caenorhabditis elegans |
